ACQUITY UPLC BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 1/pk

The ACQUITY UPLC BEH C18 Column is an advanced solution for ultra-high-performance liquid chromatography (UPLC) applications, designed to meet the rigorous demands of modern analytical laboratories. With a particle size of 1.7 µm and dimensions of 2.1 mm x 50 mm, this column offers unparalleled efficiency and precision. Its small particle size enables superior resolution and fast analysis times, making it an ideal choice for applications requiring high throughput and detailed separations. Analytical chemists and researchers rely on this column for its ability to deliver sharp peaks and reproducible results across a wide range of analytes.

One of the standout features of the ACQUITY UPLC BEH C18 Column is its wide pH compatibility, spanning from pH 1 to 12. This broad range is made possible by its trifunctionally bonded bridged ethyl hybrid (BEH) particles, which enhance its chemical stability under both acidic and basic conditions. This versatility allows the column to accommodate a diverse array of sample types, including small molecules, peptides, and proteins, making it an indispensable tool for pharmaceutical development, environmental testing, and food quality analysis. Researchers can seamlessly transition between applications without the need for multiple columns, saving both time and resources.

The column"s low bleed characteristics are optimized for sensitive detection techniques, such as mass spectrometry (MS), where minimal interference is critical. Its low bleed ensures high signal-to-noise ratios, enhancing the accuracy and reliability of MS-based analyses. Furthermore, the column"s robust construction allows it to perform consistently under the high pressures and elevated temperatures required for demanding UPLC workflows. This stability reduces the risk of column degradation, providing laboratories with a durable solution that minimizes downtime and replacement costs.

With its C18 stationary phase, the ACQUITY UPLC BEH C18 Column delivers strong retention for a variety of compounds, ranging from nonpolar to moderately polar and even some polar analytes. This versatility supports its use in numerous industries, including pharmaceutical research, environmental monitoring, and food and beverage testing. From separating complex biomolecules in protein analysis to detecting trace contaminants in environmental samples, the column excels in delivering reliable and reproducible results.

The ACQUITY UPLC BEH C18 Column is a powerful, versatile tool for chromatographers who demand high performance and efficiency. Its combination of high resolution, broad pH range, low bleed, and exceptional stability makes it a trusted choice for a wide range of applications. Whether used for drug discovery, quality control, or advanced research, this column consistently provides the precision and reliability needed to achieve analytical excellence.

FAQs About ACQUITY UPLC BEH C18 Column

1. What types of analytes can be separated using this column?
The ACQUITY UPLC BEH C18 Column is designed for a wide range of analytes, including peptides, proteins, and small molecules. Its C18 stationary phase offers strong retention for nonpolar compounds while accommodating moderately polar and some polar analytes, making it versatile for applications in pharmaceuticals, environmental testing, and food and beverage analysis.

2. What is the pH range compatibility of the ACQUITY UPLC BEH C18 Column?
This column is compatible with a broad pH range from 1 to 12, thanks to its trifunctionally bonded bridged ethyl hybrid (BEH) particle technology. This allows it to perform under acidic and basic conditions, offering flexibility for diverse sample types and analytical methods.

3. How should the ACQUITY UPLC BEH C18 Column be stored and maintained for optimal longevity?
To ensure longevity, the column should be stored in the recommended storage solvent, typically a mixture of acetonitrile and water. Avoid prolonged exposure to extreme pH or temperature conditions outside its specified range. Regular flushing with an appropriate solvent can help prevent residue buildup and maintain performance.

4. What is the benefit of BEH technology?
BEH stands for "bridged ethyl hybrid," a unique particle technology used in the ACQUITY UPLC BEH C18 Column. It combines silica and organic components to create a hybrid particle with enhanced chemical and thermal stability. This innovation allows for a wider pH range (1–12), increased column longevity, and improved performance under demanding UPLC conditions, making BEH a cornerstone of high-performance chromatography.