ACQUITY UPLC BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 1/pk
Get unprecedented levels of efficiency, peak asymmetry and chemical stability when you shop for lab equipment with ACQUITY UPLC BEH C18 Columns. The 1.7 µm ACQUITY UPLC BEH C18 Columns are a universal C18 column choice, suitable for a diverse range of analytes. These trifunctionally-bonded alkyl columns provide your lab with exceptional low- and high-pH stability between pH 1-12 — enabling you to impact the retention, selectivity, and sensitivity of ionizable compounds.
With UPLC Technology, your lab can improve resolution, sensitivity and analysis speed without compromise. Whether your separation goal is to achieve ultra-fast analysis, increase throughput while maintaining resolution, achieve ultra-high resolution while decreasing analysis time, the flexibility of the ACQUITY UPLC Solution enables you to meet method requirements.
The BEH Technology particles in ACQUITY UPLC BEH C18 VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk enable your lab to achieve excellent peak shape and efficiency for basic analytes, a rational array of chromatographic selectivity, and chemical stability improvements at mobile phase extremes, particularly at elevated pH.
With the proprietary Waters endcapping process in the ACQUITY UPLC BEH C18 Column, your lab will benefit from optimal peak shapes for bases, bringing about the highest efficiency.
FAQs About ACQUITY UPLC BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1/pk
1. What are the typical applications for the ACQUITY UPLC BEH C18 Column?
This column is versatile and can be used for a broad spectrum of analytes, including pharmaceuticals, small molecules, peptides, and other biomolecules. Its wide pH stability range makes it an excellent choice for method development, as it can handle both acidic and basic compounds. Additionally, it is often employed in various industries such as pharmaceutical, biotechnology, environmental, and food and beverage testing.
2. What is the pH range of this column?
The ACQUITY UPLC BEH C18 Column boasts a wide pH range of 1 to 12. This extensive pH range allows for flexibility in method development and the analysis of compounds that may require extreme pH conditions.
3. What are the pressure limitations?
The maximum operating pressure for this column is 15,000 psi (1034 bar). This high-pressure tolerance makes it suitable for UHPLC applications, enabling fast separations without compromising on resolution or efficiency.
4. How does a C18 stationary phase work?
In a reversed-phase chromatography system, the C18 stationary phase interacts with the non-polar regions of analytes. When a sample mixture is introduced into the column, components with higher hydrophobicity will interact more strongly with the C18 chains and thus elute later than less hydrophobic compounds. This differential retention allows for the separation of components based on their hydrophobic characteristics.
5. What is UPLC?
UPLC, which stands for Ultra Performance Liquid Chromatography, represents a significant advancement in separation science beyond HPLC (High Performance Liquid Chromatography). Utilizing UPLC columns packed with smaller particles ranging from 1.7 to 1.8 µm, this technology empowers laboratories to achieve unparalleled levels of chromatographic performance. By leveraging UPLC, labs can enhance resolution, sensitivity, and speed, all without making any compromises.
6. What is BEH?
The acronym BEH represents Ethylene Bridged Hybrid, which refers to a crucial component in UPLC Technology. The 1.7 µm BEH particle plays a significant role in enabling this technology. It comes in three distinct pore sizes (130Å, 200Å, and 300Å) and offers various bonded phases suitable for reversed-phase and hydrophilic interaction chromatography. Its applicability extends from small molecule analysis to large biopharmaceutical analysis.
