Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 186002353 ACQUITY UPLC BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk
As the premier column for method development, the 1.7 um ACQUITY UPLC BEH C18 sorbent provides you with the most universal column choice for UPLC separations. The trifunctionally bonded BEH particle gives you the widest usable pH range (pH 1-12), superior low pH stability, and ultra-low column bleed for high sensitivity MS applications.
ACQUITY UPLC BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk
At the forefront of the LC column market, the ACQUITY UPLC column family provides high-quality chromatographic information in less time than columns of the past. With all the benefits of ACQUITY UPLC columns, the BEH C18 column makes use of Ethylene Bridged Hybrid particles, one of the key enablers behind Waters' UPLC technology. These UPLC columns offer superior levels of peak asymmetry, efficiency, and chemical stability to scientists seeking versatile and high-performance separation columns.
With a must-have, ultra-low system volume, the ACQUITY UPLC BEH C18 column lab equipment also offers a fast and sensitive low-volume detector, intelligent software, negligible carryover, and the ability to run reliably higher backpressure generated by small particles operated at optimal linear velocity. These features allow chromatographers to realize the full power and promise of these sub-2 µm particle columns.
Whether you are looking for fast separations in stability monitoring, cleaning protocol validation, early drug development screening, or a number of other applications, you'll realize both speed and efficiencies through the ACQUITY UPLC BEH C18 Column as a result of the reduced column length in combination with reduced particle size by the same ratio. This balance allows the resolving power of the column and the resolution or peak capacity of the resulting chromatogram to remain intact after analysis.
In addition to the speed benefits, scientists using these columns will see ultra-high-resolution separations and consistent column-to-column performance as a result of Waters' carefully crafted length to particle size ratio. The column's dimensions also offer higher sensitivity, consume less mobile phased, and can be more easily thermostatically controlled.
What is the recommended pH range for using the ACQUITY UPLC BEH C18 Column?
The ACQUITY UPLC BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, is designed to operate over a wide pH range, from pH 1 to 12. This broad pH range allows for the analysis of a wide variety of compounds, providing flexibility in method development and robustness in handling different sample types.
What mobile phase conditions are best suited for the ACQUITY UPLC BEH C18 column?
For optimal performance with the ACQUITY UPLC BEH C18 Column, it is recommended to use a mobile phase that matches the chemical properties of the analytes being separated. Common mobile phases include water with acetonitrile or methanol, often with an acidic modifier like formic acid or trifluoroacetic acid to improve peak shape and resolution.
What types of detectors are compatible with this column?
The ACQUITY UPLC BEH C18 Column is compatible with a wide range of detectors commonly used in UPLC systems. These include UV/Vis detectors, photodiode array (PDA) detectors, fluorescence detectors, and mass spectrometers (MS).
What are the benefits of the 130Å pore size for different applications?
The 130Å pore size of the ACQUITY UPLC BEH C18 Column offers several benefits, especially for the analysis of moderately large molecules such as peptides and small proteins. This pore size provides an optimal surface area for interactions between the stationary phase and analytes, leading to improved resolution and peak shapes. It also enhances the column's ability to separate complex mixtures with high efficiency. For small molecule analysis, the 130Å pore size still provides excellent performance, making this column versatile for a broad range of applications across different fields, including pharmaceuticals, biotechnology, and environmental sciences.
Can UPLC columns be used for HPLC?
ACQUITY UPLC columns are designed to facilitate easy transfer from HPLC applications with a seamless transition. The system is known for being highly flexible, preventing the need to switch between UPLC and HPLC mode due to the same unit being capable of both. There is no need for modification or upgrades between the two.
However, UPLC columns were not created by simply adding to the existing HPLC design as typical HPLC volumes and pressure limits would impede UPLC columns. While UPLC can be used in place of HPLC columns, the reverse is not true as UPLC are far more technologically advanced than any other columns on the market.
What is HPLC?
HPLC, an acronym for high-performance liquid chromatography, represents an analytical method employed to segregate, distinguish, and measure constituents within a blend. Esteemed as the foremost technique extensively embraced across laboratories globally, this assay serves multifarious objectives.
