ACQUITY UPLC BEH C8 Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1/pk
The ACQUITY UPLC BEH C8 Column, 130Å, 1.7 µm, 2.1 mm x 100 mm, 1/pk, is an advanced analytical chromatography column designed for the separation and analysis of complex mixtures of compounds.
The ACQUITY UPLC BEH C8 Column features C8 chemistry, referring to the octadecyl stationary phase bonded to the inner surface of the column. This 8-carbon chain stationary phase is crucial for interacting with sample components, enabling effective separation. The column operates using reversed-phase chromatography, where the stationary phase is more polar than the mobile phase. In this mode, more polar analytes interact more with the stationary phase, leading to longer retention times and better separation efficiency.
One of the standout attributes of the ACQUITY UPLC BEH C8 Column is its hybrid particle substrate. This hybrid organic-inorganic material offers several advantages, including high efficiency, excellent mechanical stability, and a broad, usable pH range. The column can operate within a pH range of 1 to 12, making it versatile enough to analyze a wide variety of samples. This wide pH range is particularly beneficial for laboratories that deal with diverse analytes requiring different pH conditions for optimal separation.
The ACQUITY UPLC BEH C8 Column is designed to withstand high pressures, with a maximum pressure capacity of 18,000 psi (1240 Bar). This high-pressure tolerance allows for faster separations, which is crucial for high-throughput laboratories. However, it is important to note that higher pressures can also lead to increased column backpressure and potential band broadening. The 1.7 µm particle size of the column strikes a balance between efficiency and pressure, offering high performance while maintaining manageable backpressure levels.
With a pore size of 130 Å, the ACQUITY UPLC BEH C8 Column provides suitable accessibility of the stationary phase to sample components. This pore size is versatile enough to handle a wide range of analytes, from small molecules to larger biomolecules, making the column suitable for diverse applications. Laboratories involved in pharmaceutical analysis, environmental analysis, food analysis, clinical research, and bioanalysis will find the ACQUITY UPLC BEH C8 Column particularly useful due to its high performance and adaptability.
The column's ability to deliver high efficiency and reliability makes it an excellent choice for ultra-performance liquid chromatography (UPLC) applications. Its robust design and high-quality materials ensure consistent performance and long-lasting durability, reducing the need for frequent replacements and maintenance. This not only enhances the efficiency of laboratory workflows but also contributes to cost savings in the long run.
Whether for pharmaceutical, environmental, food, clinical, or bioanalytical research, the ACQUITY UPLC BEH C8 Column stands out as a top-tier choice for UPLC applications.
FAQs about ACQUITY UPLC BEH C8 Column
What are the main applications of the ACQUITY UPLC BEH C8 Column?
The ACQUITY UPLC BEH C8 Column is designed for UPLC applications requiring less hydrophobic retention than C18 columns, making it suitable for the fast, efficient separation of moderately non-polar compounds.
How does the BEH technology in the ACQUITY UPLC BEH C8 Column improve chromatographic performance?
BEH technology in the ACQUITY UPLC BEH C8 Column provides enhanced column stability and peak shape, particularly for basic compounds, across a wide pH range due to the ethylene-bridged hybrid particles.
Can the ACQUITY UPLC BEH C8 Column be used with high aqueous mobile phases?
Yes, the ACQUITY UPLC BEH C8 Column performs well with high aqueous mobile phases, which is beneficial for the retention and separation of polar analytes.
What are the advantages of the 1.7 µm particle size in the ACQUITY UPLC BEH C8 Column?
The small particle size provides high-resolution separations with reduced run times and improved sensitivity, beneficial for complex sample matrices or when analyte concentrations are low.
How should the ACQUITY UPLC BEH C8 Column be maintained to ensure longevity?
Regular flushing with appropriate solvents to remove any residual samples or contaminants and following the manufacturer's guidelines for column storage are recommended to maintain the column's performance.
