Chemistry |
C8 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
130 Å |
Format |
Column |
Surface Area |
185 |
System |
HPLC |
Particle Technology |
BEH |
USP Classification |
L7 |
Inner Diameter |
4.6 mm |
Length |
150 mm |
Carbon Load |
13 % |
UNSPSC |
41115709 |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge BEH C8 Column, 130Å, 5 µm, 4.6 mm X 150 mm, 1/pk
With the importance of reproducible column performance at the top of mind, Waters XBridge BEH C8 Columns are made to improve method reliability, using a tightly controlled manufacturing process to retain quality and reproducibility. XBridge columns are designed to maximize productivity, whether you are creating a quality control method or developing leading-edge LC/MS assays, the XBridge BEH C18 Columns offer a range of benefits in HPLC separations, making it a popular form of lab equipment across industries.
As the benchmark for LC method ruggedness and longevity, XBridge columns are designed to offer superior pH stability over the widest possible pH range (1-12), along with high efficiencies and a symmetrical peak shape. Scientists globally trust XBridge BEH C8 Column chemistries to provide consistently accurate results for rigorous assays. The addition of BEH Technology synthesis creates particles that ensure even more extreme column performance and longer lifetimes under harsh operating conditions. The BEH particle is specifically crafted to improve chromatographic performance of base particles and offers a range of benefits over conventional silica-based particles, including the ability to control the silanol activity with great precision.
XBridge BEH C8 Columns offer less retention than XBridge BEH C18 Column, 130Å, 3.5 µm, 2.1 mm X 150 mm, 1/pk, offering speed that may be preferred by methods developers, while still allowing for improved peak shape and chromatographic performance. As a trifunctionally bonded sorbent, XBridge BEH C8 sorbent offers the widest possible pH range, superior stability, and ultra-low column bleed.
In order to prevent revalidation of your method that is cost and time prohibitive, each batch of sorbent is tested thoroughly and all final column products are similarly evaluated. This ensures the most reproducible columns available.
How Are Guard Columns Used With XBridge Columns?
A Waters guard column of matching chemistry and particle size to the injector and main column should always be used. It is also important to use a high-performance matching guard column in order to protect the main column without compromising or changing the analytical resolution. Guard columns should be replaced at regular intervals determined by sample contamination. When system backpressure steadily increases above a set pressure limit, this is an indication that the guard column should be replaced. Sudden occurrences of split peaks also indicates a need to replace guard columns.