XBridge BEH C18 Column, 130Å, 3.5 µm, 4.6 mm X 100 mm, 1/pk

Get superior low and high pH stability with the XBridge BEH C18 Columns to achieve outstanding chromatographic peaks. Utilizing the Xbrige proprietary trifunctional bonding and end-capping the XBridge BEH C18 Columns overcome the low-pH instability caused by the siloxane bond cleavage via acid hydrolysis and reduce the rate of hydrolysis instead. This results in low retention loss when compared to columns packed with monofunctional silanes phases. This holds true even under accelerated low-pH test conditions.

XBridge BEH C18 Column particles employ ethylene bridges within the silica matrix which resist particle dissolution, overcoming the problems caused by failure of silica-based chromatographic particles at elevated pH that result in column voids, efficiency loss, and peak degradation.

Leverage unrivaled methods development flexibility with the XBridge BEH C18 Columns. Manufactured in the cGMP, ISO9001, and ISO 13485 certified facilities where every step is closely monitored for quality, the XBridge BEH C18 Columns offer outstanding reproducibility year after year, eliminating the need to test every new batch of columns and preserving long-established methods. Get consistent column performance and reliable results with the XBridge BEH C18 Columns.

You may also be interested in other lab equipment such as the XBridge BEH C8 Column 130Å, 3.5 µm, 1 mm X 50 mm, 1/pk , similar to the product listed here but suitable for analysis that requires a smaller 1mm internal diameter and 50mm column length.

Shop for lab equipment and see the complete available range of Waters Xbridge BEH Columns, and explore our collection of impressive lab equipment for your work.

FAQs About XBridge BEH C18 Column, 130Å, 3.5 µm, 4.6 mm X 100 mm, 1/pk

1. What is the optimal pH range for using the XBridge BEH C18 Column?
The XBridge BEH C18 Column is designed to operate effectively across a wide pH range of 1 to 12. This flexibility allows users to tailor their methods to the specific chemical properties of their analytes and mobile phases. The column's BEH particle technology ensures stability under both acidic and basic conditions, making it ideal for applications that require robust and versatile performance. Choosing the appropriate pH for your separation can enhance peak shape, resolution, and overall chromatographic performance.

2. What flow rates are recommended for maximizing resolution with this column?
For the XBridge BEH C18 Column, the recommended flow rate depends on the mobile phase and operating pressure, but it generally falls within 0.5 to 1.5 mL/min for optimal resolution in standard HPLC systems. Lower flow rates can enhance resolution for complex separations, while higher flow rates may be used for faster analyses, provided the system pressure does not exceed the column's limits. It's important to balance flow rate with column backpressure and separation efficiency for the best performance.

3. How should I store the XBridge BEH C18 Column when not in use?
To ensure the longevity and performance of the XBridge BEH C18 Column, it is essential to store it properly. When not in use, flush the column with an appropriate organic solvent, such as 60% acetonitrile or methanol in water, to remove the residual mobile phase and prevent microbial growth. Always cap the ends of the column securely to prevent drying out.
For long-term storage, ensure the column is stored in its original storage solvent, typically 100% organic solvent, and keep it in a cool, dry place away from direct sunlight or temperature extremes.