Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 186003034 XBridge BEH C18 Column, 130Å, 3.5 µm, 4.6 mm X 150 mm, 1/pk
XBridge BEH C18 Columns | 4.6mm X 150mm | 186003034
As a workhorse for HPLC method development, XBridge BEH C18 sorbent can be used over an entire range of mobile phase pH (1-12). The trifunctionally bonded C18 ligand provides you with a universal column for most HPLC separations and gives you the widest usable pH range, superior low pH stability, and ultra-low column bleed.
XBridge BEH C18 Column, 130A, 3.5 µm, 4.6 mm X 150 mm, 1/pk
Designed to help you maximize productivity, XBridge HPLC columns enable laboratories to overcome the most demanding chromatographic challenges. Whether you are planning to shop for lab equipment, looking for seamless transferability to UPLC, or preparative scaling for product isolation, the versatility of the XBridge BEH C18 Column is built with your major concerns in mind.
The XBridge range of columns are designed to contain the most chemically stable chromatographic sorbent on the market in order to allow exploration of the full benefits of a wide pH mobile-phase range. This chemical stability, specifically for the extremes of pH, is built into the particle during the synthesis process. This can not be duplicated using other, conventional silica-based bonding processes. While no other column can match the chemical stability offered by an XBridge column, they are able to offer increased lifetimes as a result of the superior pH stability.
The rigorous testing and validation performed in the final method can often lead to high costs for a laboratory. Each batch of sorbents and columns produced by Waters is thoroughly tested so your columns are reproducible and you do not need to revalidate any methods. These processes leave you assured that your process will be repeatable for the lifespan of your assay.
The BEH particle used in these columns is a superior technology to other silica-based particles, offering the ability to control the silanol activity with great precision. In turn, you can control and reduce any unwanted silanol interactions that may increase peak tailing. This unmatched peak shape and peak capacity will help you to maximize particle efficiency and overall productivity.
Each batch of XBridge materials including the XBridge BEH C18 Method Validation Kit, 130Å, 3.5 µm, 4.6 mm X 150 mm, 3/pk, is tested chromatographically with acidic, basic, and neutral analytes. These results are held to stringent standards to ensure you receive an excellent and reliable product every time.
FAQs About XBridge BEH C18 Column, 130Å, 3.5 µm, 4.6 mm X 150 mm, 1/pk
What types of applications is the XBridge BEH C18 Column best suited for?
This column is particularly well-suited for small molecule separations in pharmaceuticals, environmental testing, and food safety analysis. Its design ensures effective separation of complex mixtures, providing reliable retention and resolution of analytes.
How does the pore size of 130Å impact the performance of the XBridge BEH C18 Column?
The 130Å pore size makes the XBridge BEH C18 Column ideal for small to medium-sized molecules. This pore size allows for optimal interaction between the stationary phase and analytes, enhancing the separation efficiency and improving peak shapes.
What are the benefits of the 3.5 µm particle size in this column?
The 3.5 µm particle size offers a balance between resolution and pressure. It provides excellent resolution for complex mixtures while maintaining manageable back pressures, making it suitable for both conventional HPLC systems and those capable of handling higher pressures.
Can the XBridge BEH C18 Column handle high pH mobile phases?
Yes, the XBridge BEH C18 Column is designed to be chemically stable at high pH values, typically up to pH 12. This stability allows for the use of a broader range of mobile phase conditions, which can be critical for improving the solubility of some analytes or altering the selectivity of the separation.
How should the XBridge BEH C18 Column be maintained to ensure longevity?
To maintain the performance of the XBridge BEH C18 Column, it is important to regularly flush it with appropriate solvents to remove any residual analytes and prevent buildup. It is also advisable to use guard columns to protect against particulate matter. Storing the column properly when not in use, ensuring that it is fully equilibrated with the starting mobile phase before each analysis, will also help prolong its life.
What is pH?
In simple terms, pH is a measure of how acidic or basic water is. The scale goes from 0-14, with 7 representing a neutral value. Anything below a 7 indicates acidity, while anything above a 7 will be a base.
pH is used as a measure of the relative amount of free hydrogen and hydroxyl ions in the water or substance. Water with more free hydrogen ions is acidic, while water with more free hydroxyl is basic. This may be impacted by chemicals in the water and can be used to show how the water changes chemically.
What is BEH Technology?
BEH Technology, a fundamental contributor to the advancements in UPLC, harnesses the power of 1.7 µm BEH (Ethylene Bridged Hybrid) particles. These specialized particles are available in diverse pore sizes and bonded phases, opening doors to both reversed-phase and hydrophilic interaction chromatography. Notably, this technology extends its capabilities to encompass the analysis of a wide spectrum, ranging from small molecules to large molecule biopharmaceuticals.
What sets BEH Technology apart is its hybrid particle design, which inherently exhibits exceptional chemical stability. This invaluable attribute grants a broader usable pH range of pH 1-12, empowering scientists with a versatile and resilient separation technique for method development and beyond.
Moreover, the adaptability of BEH particle technology is further amplified by its availability in various HPLC particle sizes, including 2.5, 3.5, 5, and 10 µm. This seamless transition between HPLC and UPLC platforms enables laboratories to seamlessly navigate between different chromatographic approaches, capitalizing on the unique advantages offered by BEH particles at every step.
