ACQUITY UPLC BEH HILIC Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1/pk

When you need to successfully improve the retention of very polar species and provide an orthogonal separation mode for mixtures of polar and ionizable compounds, Hydrophilic Interaction Chromatography (HILIC) is a complementary and preferable chromatographic technique. HILIC uses an acetonitrile-rich, low-aqueous mobile phase in combination with a polar stationary phase in order to elute analytes in order of increasing polarity or hydrophilicity.

For these separations, the ACQUITY UPLC BEH HILIC Column makes use of unbonded BEH particles to improve retention in very polar basic analytes which can be difficult to retain through reversed-phase chromatography while demonstrating complementary selectivity to RPLC. The addition of BEH to the HILIC lab equipment offers exceptional chemical stability and peak shape when compared with silica-based HILIC stationary phases. Because of the more rugged nature of HILIC phases, this chemical stability also leads to extended column lifetimes.

As a part of the ACQUITY UPLC product family, the ACQUITY UPLC BEH HILIC Column offers the benefits of sub-2 µm particle technology, overcoming operation limits with a pressure limit of 15,000 psi (1000 bar). Holistically designed to improve resolution, sample throughput, and sensitivity, ACQUITY UPLC columns offer the unique ability to avoid switching between chromatography modes.

The ACQUITY UPLC BEH HILIC Column can also be used with the ACQUITY UPLC BEH HILIC VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk for extended column lifetimes and reduction of contaminants in the column. Each batch of columns is manufactured using material that is tested chromatographically with acidic, basic, and neutral analytes, with the results held to narrow specification ranges, ensuring excellent and reproducible performance.