Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 186003461 ACQUITY UPLC BEH HILIC Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1/pk
ACQUITY BEH HILIC columns contain unbonded BEH substrate and are designed to retain and separate very polar compounds. These unique columns are optimized and tested to produce efficient, reproducible separations under HPLC HILIC conditions. More rugged than silica based HILIC phases, the BEH particle’s chemical stability results in long column lifetimes.
ACQUITY UPLC BEH HILIC Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1/pk
When you need to successfully improve the retention of very polar species and provide an orthogonal separation mode for mixtures of polar and ionizable compounds, Hydrophilic Interaction Chromatography (HILIC) is a complementary and preferable chromatographic technique. HILIC uses an acetonitrile-rich, low-aqueous mobile phase in combination with a polar stationary phase in order to elute analytes in order of increasing polarity or hydrophilicity.
For these separations, the ACQUITY UPLC BEH HILIC Column makes use of unbonded BEH particles to improve retention in very polar basic analytes which can be difficult to retain through reversed-phase chromatography while demonstrating complementary selectivity to RPLC. The addition of BEH to the HILIC lab equipment offers exceptional chemical stability and peak shape when compared with silica-based HILIC stationary phases. Because of the more rugged nature of HILIC phases, this chemical stability also leads to extended column lifetimes.
As a part of the ACQUITY UPLC product family, the ACQUITY UPLC BEH HILIC Column offers the benefits of sub-2 µm particle technology, overcoming operation limits with a pressure limit of 15,000 psi (1000 bar). Holistically designed to improve resolution, sample throughput, and sensitivity, ACQUITY UPLC columns offer the unique ability to avoid switching between chromatography modes.
The ACQUITY UPLC BEH HILIC Column can also be used with the ACQUITY UPLC BEH HILIC VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk for extended column lifetimes and reduction of contaminants in the column. Each batch of columns is manufactured using material that is tested chromatographically with acidic, basic, and neutral analytes, with the results held to narrow specification ranges, ensuring excellent and reproducible performance.
What Is An Orthogonal Separation?
Orthogonality refers to alternative selectivity between separations in chromatography. In 2-dimensional liquid chromatography separations, they can be considered orthogonal when the two separation mechanisms used are independent of each other, providing complementary selectivities. When this occurs, the sample components will be spread out through two different retention patterns and across a broad range of retention factors.
In order to achieve a successful orthogonal separation, a suitable mobile and stationary phase are selected and take into account the properties of the sample components, including size and charge, hydrophobicity, and polarity. It can help to address major concerns like method development and insufficient resolution.
