Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 186003538 ACQUITY UPLC HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 50 mm, 1/pk
The universal, silica-based bonded phase used for the ACQUITY HSS T3 sorbents is compatible with 100% aqueous mobile phase and should be your first choice when developing separations for polar and non-polar compounds. The ACQUITY HSS T3 UPLC columns exhibit ultra-low MS bleed and are fully scalable and transferable to HPLC separations.
ACQUITY UPLC HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 50 mm, 1/pk
You’ve found the new standard in technology for retaining and separating polar compounds via reversed-phase HPLC and UPLC technology. The ACQUITY HPLC HSS T3 columns are silica-based, reversed-phase columns that retain and separate highly polar compounds. This is done with superior performance across a wider pH range, while also being certified to use in UPLC separations.
The High Strength Silica (HSS) particle was created by Waters as a pressure-tolerant, UPLC certified particle. The first of its kind, it is 100% silica and designed specifically for 15,000 psi/1000 bar applications. By utilizing the Waters advanced T3 bonding process, the ACQUITY HPLC HSS T3 columns utilize a trifunctional c18 alkyl phase that is bonded at a ligand density that promotes polar compound retention and aqueous mobile phase compatibility. Much more effective than a traditional trimethyl silane (TMS) end-capping process, Waters uses a proprietary T3 end-capping process, a primary component of many of our premier lab equipment products. This unique combination enhances performance, lifetime, peak shape, and stability.
The ACQUITY UPLC HSS particle used in these columns is not an HPLC particle, as high pore HPLC particles don’t have the mechanical stability required to withstand high column packing and operating pressures of UPLC technology. As a UPLC certified silica particle, Waters’ HSS particle is designed specifically to be rugged enough to tolerate the demanding operating conditions present in a UPLC separation. The T3 bonding method allows seamless transfer from HPLC to UPLC methods, with only slight modifications or optimization required.
Every batch of ACQUITY HSS material is tested with acidic, basic, and neutral analytes, with results held to extremely narrow specification ranges and guarantee reproducible performance. Using the ACQUITY UPLC HSS T3 column, chromatographers can benefit from superior polar and non-polar compound retention, aqueous mobile phase compatibility, an ultra-low MS bleed, and additional selectivity choices for UPLC separations.
This product is also available in similar sizes, and pairs well with many other related products, such as the Reversed-Phase QC Reference Material.
What is HSS?
HSS, or High Strength Silica particles, were developed by material scientists at Waters in order to overcome the mechanical limitations of traditional high pore volume HPLC particles. These particles do not have the necessary mechanical stability to tolerate the high pressures that occur during a UPLC separation. HSS was developed out of a desire for a silica particle that could provide high mechanical stability and the appropriate morphology to provide both long column lifetimes and UPLC efficiencies.
At 1.8-µm, the HSS particle is the first and only 100% silica particle that is designed, tested, and intended to be used in applications up to 18,000 psi.
What is UPLC?
UPLC stands for Ultra Performance Liquid Chromatography. It is the next step in separation science beyond HPLC, or High Performance Liquid Chromatography. UPLC Columns are packed with smaller particles [1.7 – 1.8-µm], enabling your lab to achieve the highest level of chromatographic performance. With UPLC Technology, your lab can improve resolution, sensitivity and speed, all without compromise.
