ACQUITY UPLC HSS T3 Column, 100A, 1.8 µm 2.1 mm X 100 mm, 1/pk
The new industry standard for retaining and separating polar compounds via reversed-phase HPLC and UPLC technology, ACQUITY UPLC HSS T3 Columns are helping to redefine compound retention. These universal silica-based, reversed-phase C18 columns can not only retain and separate highly polar compounds, but provide excellent performance across a wide range of pH. Certified for use in UPLC separations, these lab equipment provide an additional UPLC chemistry choice.
The High Strength Silica (HSS) particle is the first and only 100% silica based particle that is designed specifically for 15,000 psi/1000 bar applications and utilizing the T3 bonding capabilities. Waters' new and advanced T3 bonding process utilizes a trifunctional T3 endcapping process that is much more effective than conventional trimethyl silane (TMS) endcapping. With this unprecedented combination of bonding and encapping, you can experience superior compound retention and aqueous compatibility.
T3 columns are designed uniquely to prolong column lifetime under low pH conditions while providing superior polar compound retention and peak shape. Performance at pH 7 is also improved as the proprietary T3 endcapping procedure reacts with more active silanols, improving peak shape for bases. This helps protect the silica substrate from dissolution, also contributing to the longer column lifetimes.
ACQUITY UPLC HSS columns offer the mechanical stability that may not be present on high pore volume HPLC particles, allowing them to withstand the high column packing and operating pressures of UPLC technology. Unique and rugged, the UPLC-certified HSS particle is made specifically to withstand demanding operating conditions in your lab's UPLC separations.
Get superior polar and non-polar compound retention, ulta-low MS bleed, aqueous mobile phase compatibility, and additional selectivity choice for UPLC selections when you choose ACQUITY UPLC HSS T3 VanGuard Pre-column, 100Å, 1.8 µm, 2.1 mm X 5 mm, 3/pk. You can also benefit from a seamless transfer from HPLC methods when choosing this column.
FAQs About ACQUITY UPLC HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 100 mm, 1/pk
1.What is HSS T3 technology?
HSS (High Strength Silica) T3 technology involves the use of high-strength silica particles that are bonded with a trifunctional C18 ligand. This technology combines the mechanical strength and stability of HSS particles with the unique T3 bonding chemistry, which provides enhanced retention and separation of polar compounds. The HSS T3 columns from Waters are specifically designed to handle high-pressure conditions in UPLC applications, ensuring robust and reliable performance.
2.How do I clean the ACQUITY UPLC HSS T3 Column?
Cleaning the ACQUITY UPLC HSS T3 Column depends on the type of contaminants present. For general cleaning, it is recommended to flush the column with a series of solvents, starting with a high-water content solvent, followed by an organic solvent such as acetonitrile.
3.Can I use the ACQUITY UPLC HSS T3 Column for gradient elution?
Yes, the ACQUITY UPLC HSS T3 Column is well-suited for gradient elution methods. Gradient elution involves gradually changing the composition of the mobile phase during the run, which helps in separating complex mixtures with components of varying polarities. The robust design and high-pressure tolerance of the HSS T3 Column make it ideal for such applications, ensuring consistent performance and reliable results.
4.How does the T3 bonding chemistry enhance the performance of this column?
The T3 bonding chemistry involves a trifunctional bonding of the C18 ligand to the silica surface, providing a balanced hydrophobicity. This enhances the column's ability to retain and separate polar compounds, which are often challenging to separate using traditional C18 columns. The T3 chemistry also offers improved peak shapes and better reproducibility for a wide range of analytes, making it highly versatile for method development.
5.What is HSS technology?
High Strength Silica, or HSS technology, was designed by Waters to address the challenges laboratories face in UPLC separations. The high pore volume of HPLC particles don't have the necessary mechanical stability to withstand high pressures during UPLC separations. To address this, Waters developed a silica particle designed specifically to have high mechanical stability and appropriate morphology for long column lifetimes and high UPLC efficiencies. The 1.8-µm HSS particle is the first and only of its kind, a 100% silica particle designed and tested to be used in applications up to 15,000 psi (1034 bar). The technology is available in multiple HPLC particle sizes to enable seamless transition between HPLC and UPLC platforms.
6.What is UPLC?
UPLC, short for Ultra Performance Liquid Chromatography, represents a groundbreaking leap forward in the field of separation science. Building upon the foundations of HPLC (High Performance Liquid Chromatography), UPLC takes chromatographic performance to unparalleled heights. By utilizing specially designed columns packed with remarkably small particles ranging from 1.7 to 1.8 µm, UPLC enables laboratories to achieve exceptional levels of resolution, sensitivity, and speed in their analyses. With UPLC Technology, researchers can push the boundaries of liquid chromatography and unlock new insights without compromising on accuracy or reliability. Get ready to experience a transformative approach that revolutionizes your scientific pursuits.
