ACQUITY UPLC HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 150 mm, 1/pk

Achieve enhanced retention of polar compounds and metabolites with reversed-phase liquid chromatography with the ACQUITY UPLC HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 150 mm, 1/pk, a low ligand density C18 column that enables analytes to readily access the pore structure of the material. Attain a balanced retention of polar and hydrophobic molecules without the need for ion-pair reagents. A universal, silica-based bonded phase used for ACQUITY HSS T3 sorbents is 100% compatible with aqueous mobile phase, making it the first choice in lab equipment for developing separations for polar or non-polar compounds.

The superior performance seen in ACQUITY UPLC HSS T3 columns is due to Waters" new and advanced T3 bonding process. The T3 bonding process makes use of a trifunctional C18 alkyl phase, bonded at a ligand density that promotes polar compound retention and aqueous mobile phase compatibility. With a proprietary end-capping process, T3 is much more effective than the conventional trimethyl silane (TMS) en-dcapping seen on other columns. The combination of bonding and end-capping allows superior polar compound retention and aqueous compatibility, all while enhancing column performance, lifetime, peak shape, and stability.

Each batch of ACQUITY HSS columns is created to be used with the Waters ACQUITY UPLC system. They are manufactured in a CGMP ISO 9001:2000 certified plant that uses ultra-pure reagents. Each batch is also tested chromatographically with acidic, basic, and neutral analytes, where the results are held to narrow specifications to ensure reproducibility and excellent performance.

ACQUITY UPLC HSS T3 columns are made to solve the common problems facing separation scientists, including retaining small, water-soluble, polar organic modules. The columns are designed specifically to retain and separate polar organic compounds. Combined with UPLC technology, robust methods can be developed faster and more accurately than other tools have allowed.

ACQUITY UPLC HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 150 mm, 1/pk

1.What is the recommended mobile phase pH range for the ACQUITY UPLC HSS T3 Column?
The ACQUITY UPLC HSS T3 Column is recommended to operate within a pH range of 2 to 8. This pH range is critical to maintaining the column's integrity and ensuring optimal performance. Operating outside of this range can lead to column degradation or reduced efficiency over time. For example, highly acidic or basic mobile phases may deteriorate the stationary phase or cause the leaching of materials, leading to a shorter column life and decreased resolution in separations.

2. What are the column dimensions and packing material of the ACQUITY UPLC HSS T3 Column?
The ACQUITY UPLC HSS T3 Column is characterized by its precise dimensions and specialized packing material. It has an internal diameter (ID) of 2.1 mm and a length of 150 mm, making it suitable for high-efficiency separations in ultra-performance liquid chromatography (UPLC).
The column is packed with 1.8 µm particles, which contribute to its ability to provide high resolution and fast separations. The smaller particle size allows for improved peak sharpness and more accurate quantification of analytes, especially in complex sample matrices.

3.What is the typical column lifetime for the ACQUITY UPLC HSS T3 Column?
The lifespan of the ACQUITY UPLC HSS T3 Column can vary based on several factors, including the sample matrix, frequency of use, and how well the column is maintained. In general, with proper care and regular maintenance, the column can handle several hundred injections before its performance starts to decline.
To extend the column's lifetime, it's crucial to use appropriate mobile phases, avoid overloading samples, and follow recommended cleaning and storage procedures. Regular monitoring of column efficiency, such as tracking changes in pressure and peak shape, will help determine when it's time to replace the column, ensuring consistent and reliable performance throughout its lifespan.