ACQUITY UPLC Peptide BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 15K, 1/pk

Confidently use the ACQUITY UPLC Peptide BEH C18 Column to overcome the analytical challenges of peptide separation. The column has been designed to work with ultra-low dispersion UPLC systems while withstanding high pressures (up to 18,000 psi/1241 bar).

Achieve improved chromatography of peptides and get symmetrical, narrow peaks for best resolution. With the ACQUITY UPLC Peptide BEH C18 Column, you can successfully separate a wide range of peptides, including large and small, acidic and basic, hydrophobic, and hydrophilic.

The Peptide Separation Technology uses synthetic particles that offer the highest quality and consistent performance. The unique chemistry of the ACQUITY UPLC Peptide BEH C18 Column means that particle structure and bonding chemistry are stable at pH 1-12 and at high temperatures.

The columns are quality-control tested with a complex protein digest peptide map that means you get consistent batch-to-batch synthetic peptide or protein digestion separation.

Protect your analytical column using the ACQUITY UPLC BEH C18 VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk. The pre-column guard is designed especially for use with the ACQUITY UPLC Peptide BEH C18 Column and is the ideal lab equipment to ensure your analytical column serves a long time. This is ensured as the column is designed to protect the analytical column from any contamination, and thus, safeguarding the purity of the analytes and results, while protecting the column.

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FAQs About ACQUITY UPLC Peptide BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 15K, 1/pk

1. What types of peptides can be analyzed with this column?
The ACQUITY UPLC Peptide BEH C18 column is designed to separate a wide range of peptides, including:
Synthetic peptides: These are peptides that are chemically synthesized in a laboratory.
Natural peptides: These peptides are derived from natural sources, such as proteins or enzymes.
Post-translationally modified peptides: These peptides have undergone modifications after translation, such as phosphorylation, glycosylation, or acetylation.
The column's high efficiency and selectivity allow for the separation of complex peptide mixtures, including those with similar molecular weights or charge states.

2. What is the recommended flow rate for optimal performance of the column?
The recommended flow rate for the ACQUITY UPLC Peptide BEH C18 column will depend on several factors, including the desired analysis time, the complexity of the sample, and the desired resolution. However, a typical flow rate for UPLC applications is in the range of 0.3-0.8 mL/min.

3. What is the pore size of the ACQUITY UPLC Peptide BEH C18 Column?
The ACQUITY UPLC Peptide BEH C18 Column typically has a pore size of 130 Å. Pore size is a critical factor in peptide separations because it directly influences the accessibility of peptides to the stationary phase.
A larger pore size (like 130 Å) allows for better penetration of larger peptides into the pores of the stationary phase. This results in improved retention, peak shape, and resolution for peptides, especially larger ones. In contrast, smaller pore sizes might limit the access of larger peptides, leading to poor peak shape and reduced retention. The 130 Å pore size of this column is specifically designed to optimize the separation of peptides, particularly those with higher molecular weights.