SKU: 186003686
ACQUITY UPLC Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 mm X 100 mm, 1K - 30K, 1/pk

ACQUITY UPLC Peptide BEH C18 Columns | 186003686


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Product Description

The ACQUITY BEH300 C18 Peptide Separation Technology (PST) columns have been specially optimized and QC tested for peptide separations. The wide-pore (300Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    12 pH

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    1000

  • Molecular Weight Range Max

    30000

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    300 Å

  • QC Tested

    Peptide

  • Format

    Column

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L1

  • Inner Diameter

    2.1 mm

  • Length

    100 mm

  • Carbon Load

    18 %

  • UNSPSC

    41115709

  • Application

    Peptide

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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ACQUITY UPLC Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 mm X 100 mm, 1K - 30K, 1/pk

Meet the challenges of the demanding peptide separation easily with the ACQUITY UPLC Peptide BEH C18 Column. The ACQUITY UPLC Peptide BEH C18 Column makes an ideal piece of lab equipment as it offers narrow, symmetrical peaks for best resolution as well as good peak shape and retention in formic acid and trifluoroacetic acid (TFA).

The ACQUITY UPLC Peptide BEH C18 Column is part of the Peptide Separation Technology column series which is based on synthetic particles that ensure the highest quality and consistent performance. These columns are also specifically QC tested with a complex protein digest peptide map. You can be certain of the stability of the particle structure and bonding chemistry at pH 1 to 12 at elevated temperatures. In addition to this, the ACQUITY UPLC Peptide BEH C18 Column offers consistent batch-to-batch synthetic peptide or protein digest separation.

Improve quantitation of modified peptides with the ACQUITY UPLC Peptide BEH C18 Column. The higher resolution and increased sensitivity offered by the ACQUITY UPLC Peptide BEH C18 Column allows modified peptides to be seen at lower levels because they are more easily separated from unmodified peptides.

Since the surface chemistry of Peptide Separation Technology (PST) columns yields sharper and more symmetrical peaks to simplify analyses, the ACQUITY UPLC Peptide BEH C18 Column is the perfect lab equipment to use for the analysis of glycoproteins and glycopeptides.

To ensure that your ACQUITY UPLC Peptide BEH C18 analytical column lasts a long time, you may want to consider purchasing the ACQUITY UPLC Peptide BEH C18 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm, 1K - 30K, 3/pk. The VanGuard pre-column is designed to be used with the analytical column and protects it by removing any chemical or particulate material from the mobile phase. In addition to this, it also results in reliable test results since any interference is removed by the column guard.

See our complete range of products to see our latest offerings. Explore related products and shop for lab equipment.

How Does Temperature Affect Method Optimization Of Peptides And Proteins?

Changing the temperature is effective for changing separation selectivity or improving peak shape. The usable temperature range is limited by column durability, however. As a general rule, strongly acidic conditions combined with heat will accelerate the elimination of functional groups. This in return will result in short retention time and/or increase the unfavorable secondary interaction between the packing material and sample.