Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Endcapped |
Yes |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
30000 |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
300 Å |
QC Tested |
Peptide |
Format |
Column |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
2.1 mm |
Length |
100 mm |
Carbon Load |
18 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 mm X 100 mm, 1K - 30K, 1/pk
Meet the challenges of the demanding peptide separation easily with the ACQUITY UPLC Peptide BEH C18 Column. The ACQUITY UPLC Peptide BEH C18 Column makes an ideal piece of lab equipment as it offers narrow, symmetrical peaks for best resolution as well as good peak shape and retention in formic acid and trifluoroacetic acid (TFA).
The ACQUITY UPLC Peptide BEH C18 Column is part of the Peptide Separation Technology column series which is based on synthetic particles that ensure the highest quality and consistent performance. These columns are also specifically QC tested with a complex protein digest peptide map. You can be certain of the stability of the particle structure and bonding chemistry at pH 1 to 12 at elevated temperatures. In addition to this, the ACQUITY UPLC Peptide BEH C18 Column offers consistent batch-to-batch synthetic peptide or protein digest separation.
Improve quantitation of modified peptides with the ACQUITY UPLC Peptide BEH C18 Column. The higher resolution and increased sensitivity offered by the ACQUITY UPLC Peptide BEH C18 Column allows modified peptides to be seen at lower levels because they are more easily separated from unmodified peptides.
Since the surface chemistry of Peptide Separation Technology (PST) columns yields sharper and more symmetrical peaks to simplify analyses, the ACQUITY UPLC Peptide BEH C18 Column is the perfect lab equipment to use for the analysis of glycoproteins and glycopeptides.
To ensure that your ACQUITY UPLC Peptide BEH C18 analytical column lasts a long time, you may want to consider purchasing the ACQUITY UPLC Peptide BEH C18 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm, 1K - 30K, 3/pk. The VanGuard pre-column is designed to be used with the analytical column and protects it by removing any chemical or particulate material from the mobile phase. In addition to this, it also results in reliable test results since any interference is removed by the column guard.
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How Does Temperature Affect Method Optimization Of Peptides And Proteins?
Changing the temperature is effective for changing separation selectivity or improving peak shape. The usable temperature range is limited by column durability, however. As a general rule, strongly acidic conditions combined with heat will accelerate the elimination of functional groups. This in return will result in short retention time and/or increase the unfavorable secondary interaction between the packing material and sample.