ACQUITY UPLC Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk
Waters ACQUITY UPLC Peptide BEH C18, wide pore (300 Å) Columns are trifunctionally bonded and offer a wide usable pH range (1-12) and exceptional low pH stability. They are critical pieces of lab equipment for any scientist as they offer ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.
Experience improved chromatography of peptides with the ACQUITY UPLC Peptide BEH Columns. Achieve narrow, symmetrical peaks for best resolution, successful separations of a wide range of peptides including large and small, acidic and basic, hydrophobic and hydrophilic. You can also be certain of good peak shape and retention in formic acid and trifluoroacetic acid (TFA) for optimal chromatography and detection.
Designed specially to be compatible with ACQUITY UPLC and ACQUITY PREMIER systems, the columns are manufactured in a cGMP, ISO 9002 certified plant using ultra-pure reagents. Each batch is tested chromatographically with acidic, basic, and neutral analytes and has been qualified for peptide mapping. To assure you get exceptional lab equipment that provides reliable analysis and reproducible performance, the results are held to narrow specification ranges.
As the ACQUITY UPLC Peptide columns utilize the Peptide Separation Technology (PST) that is based on the BEH Technology particles, they meet the demanding specifications for the BEH Technology particles and are specifically QC certified with a peptide map. This results in the stability of well-developed peptide separations and predictable behavior with many different samples used in the proteomics, protein characterization, and peptide synthesis. The surface chemistry gives sharp symmetrical peaks over a wide range of peptide properties and application requirements.
The ACQUITY UPLC Peptide BEH C18 column is also available in a 130 Å variation and can be used effectively alongside the ACQUITY UPLC Peptide BEH C18 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm, 1K - 30K, 3/pk to help you achieve optimal peptide separations regardless of pore size.
FAQs About ACQUITY UPLC Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 30K, 1/pk
1. How does the BEH particle technology benefit peptide analysis?
The BEH (Ethylene Bridged Hybrid) particle technology used in the ACQUITY UPLC Peptide BEH C18 Column offers exceptional stability under high pressures and a wide pH range (1-12), making it ideal for peptide analysis. This advanced technology ensures consistent performance, minimizes column degradation, and maintains peak shape even under demanding conditions. Its robustness also supports reproducible separations and high-resolution analysis, enabling accurate peptide mapping and characterization across a variety of workflows.
2. Is the column compatible with high-temperature workflows for peptide analysis?
Yes, the ACQUITY UPLC Peptide BEH C18 Column is compatible with high-temperature workflows, supporting temperatures up to 80°C. Elevated temperatures improve peptide separations by enhancing analyte diffusion and reducing retention times, which can be especially beneficial for complex peptide mixtures. The BEH particle technology ensures the stability and longevity of the column under these conditions, making it suitable for high-performance peptide analyses requiring precision and efficiency.
3. What is the molecular weight range supported by this column?
The ACQUITY UPLC Peptide BEH C18 Column is specifically designed for the separation of peptides within a molecular weight range of 1,000 to 30,000 Daltons. Its 300Å pore size provides ample space for larger peptides to access the stationary phase, ensuring efficient separations and sharp peak shapes for both small and large peptides. This broad range makes it a versatile tool for peptide mapping, characterization, and biomolecule analysis.
