Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Bonding Technology |
T3 |
Silanol Activity |
Medium |
Particle Shape |
Spherical |
Particle Size |
3 µm |
Endfitting Type |
Waters |
Pore Size |
100 Å |
Format |
Column |
Surface Area |
330 |
System |
HPLC |
USP Classification |
L1 |
Inner Diameter |
4.6 mm |
Length |
150 mm |
Carbon Load |
14 % |
UNSPSC |
41115709 |
Brand |
Atlantis |
Product Type |
Columns |
Units per Package |
1 pk |
FAQs about Atlantis T3 Column
What makes the Atlantis T3 Column optimal for the separation of polar compounds?
The Atlantis T3 Column is designed with a proprietary T3 bonding technology that enhances retention of polar compounds, making it particularly effective for reversed-phase chromatography where polar compound retention is often challenging.
How does the particle size in the Atlantis T3 Column affect its chromatographic performance?
The 3 µm particle size in the Atlantis T3 Column enables high-resolution separations with reduced run times and solvent consumption, which is advantageous for both routine and complex analyses.
Is the Atlantis T3 Column compatible with both HPLC and UPLC platforms?
The Atlantis T3 Column is designed to be compatible with HPLC systems, and due to its small particle size, it may also be used on UPLC platforms, provided the system can operate at the pressures required for smaller particles.
Can the Atlantis T3 Column be used for the analysis of biomolecules?
Yes, the Atlantis T3 Column's pore size and surface chemistry are suitable for the analysis of small to medium biomolecules, offering good peak shape and resolution.
What are the best practices for maintaining the Atlantis T3 Column?
Maintaining the Atlantis T3 Column involves using appropriate cleaning protocols with solvents that are compatible with the column's bonding chemistry and storing the column under recommended conditions to prevent any degradation of performance.
What is a ligand?
Ligands are ions or neutral molecules that bond to a central metal atom or ion to form a coordination complex. A ligand acts as a Lewis base (electron pair donor), and the central atom acts as a Lewis acid (electron pair acceptor). Ligands all have at least one donor atom with an electron pair, which is used to form covalent bonds with the central atom. The size of a ligand is dictated by its cone angle. Ligands can be anions, cations, or neutral molecules. They can also be characterized as monodentate, bidentate, tridentate, etc.
What is HPLC?
High-performance liquid chromatography (HPLC) is an advanced analytical method employed to effectively segregate, discern, and measure constituents within a complex amalgamation. As the predominant technique embraced across laboratories worldwide, this test assumes paramount significance, catering to a diverse range of objectives with unparalleled versatility.