Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
130 Å |
QC Tested |
Peptide |
Format |
OBD Prep Column |
Surface Area |
185 |
System |
HPLC |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
19 mm |
Length |
150 mm |
Carbon Load |
18 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
XBridge |
Product Type |
Preparative Columns |
Units per Package |
1 pk |
XBridge Peptide BEH C18 OBD Prep Column, 130Å, 5 µm, 19 mm X 150 mm, 1/pk
Waters Peptide Separation Technology (PST), which combines BEH Technology particles, is used in the XBridge Peptide BEH C18 OBD Prep Columns. Peptide Separation Technology is a set of chromatographic techniques that can be used in any research and development application that requires peptide analysis and isolation. Peptide Separation Technology columns are made of C18 BEH Technology particles and come in sizes ranging from 1.7 to 10 meters. Column diameters range from 75 to 300 m, with lengths ranging from 50 to 250 mm. All particle sizes, in either 130A or 300A pore diameters, have a surface chemistry that produces sharp, symmetrical peaks throughout a wide range of peptide characteristics and application requirements. This specific listing has a diameter of 19mm, length of 150mm, and supports a carbon load of 18%.
These columns have been thoroughly validated with a peptide map to ensure that scientists will get stable peptide separation methods as well as predictable behavior over a wide range of concentrations, including proteomics, protein characterization, and peptide synthesis.
The column's injected BEH Technology will improve the resolution of complex peptide digests. The XBridge Peptide BEH C18 OBD Prep Column provides good peptide separation over a broad array of peptides. For optimal chromatography, it will also give you superior peak shape and retention in formic acid and trifluoroacetic acid. The XBridge Peptide BEH C18 OBD Prep Columns also allow for seamless technique migration from sub-2 m UPLC Technology to 5 m preparative HPLC separations.
Find various products that are compatible with the XBridge Peptide BEH C18 OBD Prep Column, including guards, vials, elution plates from our website. The website enables you to shop for lab equipment, as well as to reach out with any queries you may have. You can direct these queries and concerns to a member of our staff, located in one of our numerous offices located globally.
You might also be interested in the XBridge Peptide BEH C18 OBD Prep Column, 130Å, 10 µm, 10 mm X 250 mm, 1/pk; which has a small-pore (130Å) tri functionally bonded BEH particle that offers you the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.
What Is The Optimal pH Range For The XBridge Peptide BEH C18 OBD Prep Column?
The XBridge Peptide BEH C18 OBD Prep Column ideally operates within the 1 - 12 pH range and can withstand a maximum pressure of 6000 psi (or 415 Bar). Please keep in mind that using higher pH may impact the performance and lifetime of your column.