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SKU: 186003953 XBridge Oligonucleotide BEH C18 Column, 130Å, 2.5 µm, 4.6 mm X 50 mm, 1/pk
Waters Oligonucleotide Separation Technology (OST) columns are based on the second generation of hybrid-silica BEH™ Technology particles functionalized with C18. The separation of detritylated synthetic oligonucleotide samples is based on the well-established method of ion-pair, reversed-phase chromatography. The availability of 1.7µm UPLC® technology or 2.5µm HPLC particles in various column dimensions provides flexibility to meet various laboratory-scale isolation or analysis needs and delivers exceptional sample resolution and superior column life. In addition, Waters manufacturing and quality control testing procedures help ensure consistent batch-to-batch and column-to-column performance regardless of application demands.
XBridge Oligonucleotide BEH C18 Column, 130Å, 2.5 µm, 4.6 mm X 50 mm, 1/pk
Based on the second generation of hybrid-silica BEH Technology particles functionalized with C18, Waters Oligonucleotide Separation Technology (OST) columns have allowed chromatographers to meet a wide variety of isolation and analytical needs. The XBridge Oligonucleotides BEH C18 Column facilitates the separation of detritylated synthetic oligonucleotide samples, based on well-established methods of ion-pair, reversed-phase chromatography, while delivering exceptional resolution and column lifetime.
When used in separations of detritylated synthetic oligonucleotide samples, XBridge Oligonucleotide BEH C18 Columns offer separation efficiencies equivalent or better than other lab equipment for ion-exchange HPLC methods. Chromatographers will also see the ability to resolve large oligonucleotide sequences due to the enhanced resolving power of sub-3 μm particles and scalable column offerings for lab scale isolation needs. Laboratories also see a reduced cost per analysis due to the exceptional lifetime of these columns.
This column has an inner diameter of 4.6 mm and a length of 50 mm, part of the wide variety of column sizes that make XBridge Oligonucleotide BEH C18 Columns the preferred choice for detritylated oligonucleotide purifications. Separation scientists have the ability to select the most appropriate size column based on the sample amount available, allowing for maximum component resolution and recovery of the target product from non-desired failure sequences.
XBridge Oligonucleotide BEH C18 Columns and XBridge PREMIER Oligonucleotide BEH C18 Column, 130Å, 2.5 µm, 4.6 mm X 50 mm, 1/pk packing material is manufactured in a cGMP, ISO 9002 certified plant using ultrapure reagents. Each batch of material is tested chromatographically with acidic, basic, and neutral analytes with results held to narrow specification ranges to assure excellent and reproducible performance regardless of application demands, across batches and columns. A copy of the Performance Test Chromatogram and Certificate of Acceptance is included with each column.
FAQs About XBridge Oligonucleotide BEH C18 Column, 130Å, 2.5 µm, 4.6 mm X 50 mm, 1/pk
1. What is the difference between the XBridge Oligonucleotide BEH C18 Column and other oligonucleotide columns?
The XBridge Oligonucleotide BEH C18 Column stands out due to its advanced BEH particle technology, which delivers superior mechanical strength and chemical stability compared to other oligonucleotide columns. This technology ensures reliable performance across a wide pH range (1-12), making it suitable for challenging analytical conditions. The column provides exceptional resolution, reproducibility, and sensitivity, enabling better peak shapes and improved detection of low-level impurities.
The robust design also reduces the need for frequent replacements, saving time and costs in routine analyses. This makes the XBridge Oligonucleotide BEH C18 Column a reliable choice for high-performance oligonucleotide separations in pharmaceutical, genomic, and analytical workflows.
2. What is the recommended flow rate for this column?
The recommended flow rate for the XBridge Oligonucleotide BEH C18 Column is generally 1 mL/min under standard HPLC conditions. However, the optimal flow rate may vary depending on factors such as the mobile phase composition, gradient profile, and the complexity of the oligonucleotide sample. Adjusting the flow rate can help fine-tune resolution and separation efficiency, particularly for complex mixtures or when analyzing closely related oligonucleotide species.
3. How should the column be stored?
Proper storage is essential to maintain the performance and longevity of the XBridge Oligonucleotide BEH C18 Column. The column should be flushed with an appropriate storage solvent, such as 100% acetonitrile, before storage to remove the residual mobile phase and prevent microbial growth. Always store the column at room temperature in a dry, temperature-stable location, away from direct sunlight and humidity fluctuations. You should also ensure both ends of the column are capped securely to prevent the stationary phase from drying out.
How Do I Minimize Band Spreading With XBridge OST C18 Columns?
Detritylated synthetic oligonucleotide separations are performed almost exclusively with gradient elution techniques. As such, the impact of pre-column sample band broadening can be minimized if you allow the sample to bind to the column prior to beginning the actual separation gradient.
However, a proper connection between the XBridge OST C18 column outlet and the detector is crucial to minimize the deleterious effect of post-column sample band spreading. Use of appropriate internal diameter tubing is recommended- for example, 0.005 inch PEEK tubing for UV detector applications.
