Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Temperature Limits |
45 C |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
No |
Bonding Technology |
C18 SB |
Silanol Activity |
High |
Particle Shape |
Spherical |
Particle Size |
1.8 µm |
Endfitting Type |
Parker-style |
Pore Size |
100 Å |
Format |
Column |
Surface Area |
230 |
System |
UHPLC, UPLC |
Particle Technology |
HSS |
USP Classification |
L1 |
Inner Diameter |
2.1 mm |
Length |
100 mm |
Carbon Load |
8.5 % |
eCord |
Yes |
UNSPSC |
41115709 |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC HSS C18 SB Column, 100Å, 1.8 µm, 2.1 mm X 100 mm, 1/pk
Leverage the unique non-end-capped, low-coverage silica-based C18 chemistry of the ACQUITY UPLC HSS C18 SB Column to get alternate selectivity for compounds that are impacted by silanophilic interactions. The lab equipment offers a unique increased silanol activity which results in the greater retention of basic compounds due to secondary interactions with residual silanols. This takes place while retention of non-basic analytes is reduced due to the low ligand density and ionic repulsion. This distinctive combination of retention characteristics makes the ACQUITY UPLC HSS C18 SB Column a powerful method development tool that can impart differences in Selectivity for Bases (SB) while delivering outstanding peak shape.
Minimize the risk of method variation that is caused by chromatographic media batch or column inconsistency. This is why you can depend on the ACQUITY UPLC HSS C18 SB Column to deliver consistent performance, as the column has been designed to give results that will be repeatable and reproducible year after year.
The packing materials of the ACQUITY UPLC HSS C18 SB Column are designed specifically for use with the ACQUITY UPLC System. Additionally, they are manufactured in a cGMP, ISO 9001 certified plant using ultra-pure reagents. Each batch of the packing material is tested chromatographically with acidic, basic, and neutral analytes, and the results are held to narrow specification ranges to assure excellent, reproducible performance.
To shop for lab equipment, to find all products that are compatible with the current listing, or to find other variants of the same listing, you can browse through our website. The website contains reference literature that explains our research and development in detail as well, to help you understand how our equipment works.
You may also be interested in the ACQUITY UPLC HSS C18 SB VanGuard Pre-column, 100Å, 1.8 µm, 2.1 mm X 5 mm, 3/pk which works in conjunction with your analytical column, and has been designed exclusively to protect and guard the column. It removes chemical and physical particles from the mobile phase, and thus, protects the analytical column from harm and interference, ensuring a long lifetime for the column, and accurate results for the analysis.
How Is the ACQUITY UPLC HSS C18 SB Column Equilibrated?
To equilibrate the column, use a minimum of 10-column volumes of the mobile phase. The column may be considered thermally equilibrated once constant backpressure is achieved.