Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 186004454 XBridge BEH HILIC Column, 130Å, 5 µm, 4.6 mm X 250 mm, 1/pk
XBridge BEH HILIC columns contain unbonded BEH substrate and are designed to retain and separate very polar compounds. These unique columns are optimized and tested to produce efficient, reproducible separations under HPLC HILIC conditions. More rugged than silica based HILIC phases, the BEH particle’s chemical stability results in long column lifetimes.
XBridge BEH HILIC Column, 130Å, 5 µm, 4.6 mm X 250 mm, 1/pk
Depend on the XBridge BEH HILIC Column to improve the retention of very polar species that are not well retained by traditional reversed-phase chromatography. The analytical column makes this possible by the unique attributes of Hydrophilic Interaction Chromatography (HILIC).
The XBridge BEH HILIC Column offers alternative selectivity to traditional reversed-phase columns in addition to providing improvements in peak shape, retention, and column lifetime when compared to silica-based HILIC columns. The robust and rugged BEH Technology on which the XBridge BEH HILIC Column is based makes the column a new standard for HILIC separations.
Get enhanced retention for polar analytes with the XBridge BEH HILIC Column. The HILIC technology used in the column offers complex retention mechanisms combining partitioning, ion exchange, and hydrogen bonding. You also get greater retention than reversed-phase as retention occurs with a polar stationary phase combined with mobile phases made up of acetonitrile concentrations greater than 80%. The analytical column also offers complimentary selectivity to reversed-phase columns. In addition to improved retentivity, reversal in elution has also been observed which is beneficial when developing orthogonal separations for polar analytes.
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You may also be interested in XBridge BEH HILIC VanGuard Cartridge, 130Å, 5 µm, 3.9 mm X 5 mm, 3/pk, which ensures that your analytical column performs at the same level throughout its lifetime. Made from the same packing material as the XBridge BEH HILIC Column, the VanGuard Cartridge is designed to protect the analytical column from any chemical and particulate material in its mobile phase. This in turn makes sure that the column continues to give excellent performance and reliable results for a long time.
What Pressure Range Can The XBridge BEH HILIC Column Tolerate?
The recommended operating pressure for the XBridge BEH HILIC Column is up to 6000 psi (400 bar or 40 Mpa). It is important to note that pressures greater than the range of 4000-5000 psi should be avoided as they can result in reduced column lifetime and decreased column performance.
