Columns for Antibody Drug Conjugates (ADC)

SKU: 186004495
ACQUITY UPLC Protein BEH C4 Column, 300 Å, 1.7 µm, 2.1 mm x 50 mm, 1/pk

Acquity UPLC Protein BEH C4 Column | 186004495

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Product Description

The ACQUITY UPLC Protein BEH C4, 300 Å, 1.7 µm, 2.1 mm x 50 mm column is QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity.

Specifications

  • Chemistry

    C4

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    2 pH

  • pH Range Max

    10 pH

  • Endcapped

    No

  • Silanol Activity

    Low

  • Molecular Weight Range Max

    150000

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    300 Å

  • QC Tested

    Protein

  • Format

    Column

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L26

  • Inner Diameter

    2.1 mm

  • Length

    50 mm

  • Carbon Load

    8 %

  • UNSPSC

    41115709

  • Application

    Protein

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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FAQs about ACQUITY UPLC Protein BEH C4 Column

What specific features of the ACQUITY UPLC Protein BEH C4 Column make it suitable for protein analysis?
The ACQUITY UPLC Protein BEH C4 Column's large pore size (300Å) and BEH (Bridged Ethyl Hybrid) technology facilitate the analysis of large biomolecules by providing a stable, wide-pore stationary phase for superior protein separations.

Is the ACQUITY UPLC Protein BEH C4 Column compatible with mass spectrometry?
Yes, the ACQUITY UPLC Protein BEH C4 Column is designed to be MS-friendly, allowing for direct coupling to mass spectrometry for detailed protein and peptide characterization.

Can the ACQUITY UPLC Protein BEH C4 Column be used for intact protein analysis?
The ACQUITY UPLC Protein BEH C4 Column is suitable for intact protein analysis, providing high resolution and peak capacity, which are essential for the characterization of complex proteins.

How does the ACQUITY UPLC Protein BEH C4 Column handle the separation of hydrophobic peptides?
The C4 bonded phase offers an optimal balance of hydrophobicity and accessibility, enabling the effective separation of hydrophobic peptides while minimizing issues like adsorption and peak tailing.

What are the best practices for maintaining the ACQUITY UPLC Protein BEH C4 Column?
To maintain the ACQUITY UPLC Protein BEH C4 Column, users should utilize appropriate washing protocols to remove retained substances and follow the manufacturer's guidelines for proper storage.

What Is Protein Separation Technology?

When analyzing and characterizing protein samples, it can be important to detect small chemical differences between large molecules. In the past, reversed-phase HPLC has not been used often in these tests due to the broad and asymmetrical peaks often seen during protein separation. By using the ethylene bridged hybrid (BEH) particle, Waters has been able to create Protein Separation Technology that is designed specifically for high resolution of protein analysis. Traditional performance limitations are overcome using a combination of the BEH particle, short-chain bonded phases, and wide pores, leading to minimal secondary interactions, long column lifetime, and minimal carryover with maximum recovery.