SKU: 186004496
ACQUITY UPLC Protein BEH C4 Column, 300 Å, 1.7 µm, 2.1 mm x 100 mm, 1/pk

Acquity UPLC Protein BEH C4 Column | 186004496

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Product Description

The ACQUITY UPLC Protein BEH C4, 300 Å, 1.7 µm, 2.1 mm x 100 mm column is QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity.

Specifications

  • Chemistry

    C4

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    2 pH

  • pH Range Max

    10 pH

  • Endcapped

    No

  • Silanol Activity

    Low

  • Molecular Weight Range Max

    150000

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    300 Å

  • QC Tested

    Protein

  • Format

    Column

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L26

  • Inner Diameter

    2.1 mm

  • Length

    100 mm

  • Carbon Load

    8 %

  • UNSPSC

    41115709

  • Application

    Protein

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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FAQs about ACQUITY UPLC Protein BEH C4 Column

How does the ACQUITY UPLC Protein BEH C4 Column enhance UPLC applications for proteins?
The ACQUITY UPLC Protein BEH C4 Column is tailored for UPLC applications, providing sharp peak shapes and fast separations for proteins and peptides, which is particularly beneficial when working with complex biological samples or when high throughput is required.

What types of biomolecules are best separated with the ACQUITY UPLC Protein BEH C4 Column?
The ACQUITY UPLC Protein BEH C4 Column is ideal for separating medium to large proteins, including monoclonal antibodies and membrane proteins, due to its large pore size and hydrophobic C4 surface.

Can the ACQUITY UPLC Protein BEH C4 Column handle high throughput screening applications?
Yes, the ACQUITY UPLC Protein BEH C4 Column is well-suited for high throughput screening applications due to its superior column strength and fast separation capabilities enabled by UPLC technology.

What are the key considerations for solvent compatibility with the ACQUITY UPLC Protein BEH C4 Column?
Solvent compatibility with the ACQUITY UPLC Protein BEH C4 Column includes the use of organic modifiers and acidic to neutral pH buffers to maintain the integrity of the biomolecules and the stationary phase during separations.

What maintenance and storage guidelines should be followed for the ACQUITY UPLC Protein BEH C4 Column?
The ACQUITY UPLC Protein BEH C4 Column should be maintained by flushing with appropriate solvents and stored in a solvent that ensures the stability of the bonded phase, such as 80% acetonitrile in water, as recommended by the manufacturer.

How Should Samples Be Prepared For Use With ACQUITY UPLC BEH C4 Columns?

Before injection into the system, samples should be free of all particles, as impurities can contribute to column contamination. It is recommended that samples are prepared in the operating mobile phase, or a mobile phase that is weaker than the mobile phase, to ensure optimal peak shape and sensitivity. If samples are not dissolved in the mobile phase, ensure that sample, solvent, and mobile phase are miscible in order to avoid sample and buffer precipitation. Filter your sample with 0.2 µm filters to remove particulates. Alternatively, centrifugation for 20 minutes at 12-50,000 g could be considered.