ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 30K, 1/pk

Using the characteristically small particle size of 1.7 μm from UPLC technology, ACQUITY UPLC Protein BEH C4 Columns are packed in a way that reduces dispersion and band broadening. With this capability, chromatographers can achieve improved resolution, sensitivity, and speed in protein separations. The combination of Waters’ Protein Separation Technology (PrST), these hybrid particles, a short-chain bonded phase, and wide pores allow these columns to overcome the performance limitations associated with traditional silica-based column packings.

Lab equipment based on PrST is able to tolerate extreme pH temperature and operating conditions while simultaneously maximizing recovery, minimizing protein carryover, and maintaining a long column lifetime. The use of ACQUITY UPLC Protein BEH C4 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm, 10K - 500K, 3/pk is recommended to protect these extended lifetime capabilities.

ACUITY UPLC Protein BEH C4 Columns contain a C4 ligand-based sorbent which offers less retention when compared to traditional C18 counterparts. This C4 ligand allows for minimization of protein carryover, increased protein recovery, and improved peak capacity all while maintaining minimal secondary interactions.

The 1.7 μm particle packing materials enable enhancements to be realized on protein separations, making ACQUITY UPLC Protein BEH C4 Columns scalable across UPLC platforms in addition to being effective with MS-compatible eluents, addressing the need for advanced detection techniques.

Packing materials for the ACQUITY UPLC Protein BEH C4 Column are manufactured in a cGMP, ISO 9002 certified plant and use ultrapure reagents. Each batch of materials undergoes qualification with a protein test mixture, ensuring a reproducible performance across laboratories.