SKU: 186004498
XBridge Protein BEH C4 Column, 300 Å, 3.5 µm, 2.1 mm x 50 mm, 1/pk

XBridge Protein BEH C4 Column | 186004498

Streamline Purchasing with Customized Quotes

Streamline Purchasing with Customized Quotes

Shop on Waters.com and add items to your cart

Navigate to the Shopping Cart

Select Request a Quote to get your quote instantly

Learn More

Product Description

The XBridge Protein BEH C4, 300 Å, 3.5 µm, 2.1 mm x 50 mm column is QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity.

Specifications

  • Chemistry

    C4

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    2 pH

  • pH Range Max

    10 pH

  • Maximum Pressure

    6000 psi (415 Bar)

  • Endcapped

    No

  • Silanol Activity

    Low

  • Molecular Weight Range Max

    150000

  • Particle Shape

    Spherical

  • Particle Size

    3.5 µm

  • Endfitting Type

    Waters

  • Pore Size

    300 Å

  • QC Tested

    Protein

  • Format

    Column

  • Surface Area

    90

  • System

    HPLC

  • Particle Technology

    BEH

  • USP Classification

    L26

  • Inner Diameter

    2.1 mm

  • Length

    50 mm

  • Carbon Load

    8 %

  • UNSPSC

    41115709

  • Application

    Protein

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

What do you want to do?

What do you want to do?
Need Help?
Back To Top Back To Top

FAQs about XBridge Protein BEH C4 Column

What are the advantages of using the XBridge Protein BEH C4 Column for protein and peptide separations?
The XBridge Protein BEH C4 Column, with its large pore size and C4 alkyl chain, is designed to provide high-resolution separations of proteins and peptides with improved recovery and minimal sample loss, which is essential for maintaining the integrity of large biomolecules during analysis.

How does the pore size of the XBridge Protein BEH C4 Column affect its use in chromatography?
The 300Å pore size of the XBridge Protein BEH C4 Column is optimized for the analysis of large biomolecules, allowing for the accommodation of proteins and peptides with high molecular weights without compromising separation efficiency.

Can the XBridge Protein BEH C4 Column be used for the analysis of hydrophobic proteins and peptides?
Yes, the C4 bonded phase of the XBridge Protein BEH C4 Column provides a hydrophobic interaction environment that is particularly suitable for the analysis of hydrophobic proteins and peptides.

Is the XBridge Protein BEH C4 Column compatible with a wide range of mobile phases?
The XBridge Protein BEH C4 Column is designed to be compatible with a wide pH range and diverse mobile phases, including organic modifiers, which facilitates flexible method development for different protein and peptide analyses.

What is the recommended procedure for maintaining the XBridge Protein BEH C4 Column?
For maintenance, the XBridge Protein BEH C4 Column should be regularly flushed with solvents to prevent build-up of non-eluted material and stored under recommended conditions to preserve the column’s performance and longevity.

What Operating pH Limits Can Work With The XBridge Protein BEH C4 Column?

The recommended operating pH range for XBridge Protein BEH C4 Column is between 1 to 12. In addition to this, it is important to note that the column lifetime will vary depending upon the operating temperature as well as the type and concentration of buffer used.