Chemistry |
C4 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
500000 |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Direct-Connect |
Pore Size |
300 Å |
QC Tested |
Protein |
Format |
VanGuard Pre-column |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
USP Classification |
L26 |
Inner Diameter |
2.1 mm |
Length |
5 mm |
Carbon Load |
8 % |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
ACQUITY UPLC |
Product Type |
Guards |
Units per Package |
3 pk |
ACQUITY UPLC Protein BEH C4 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm, 10K - 500K, 3/pk
Invest in the ACQUITY UPLC Protein BEH C4 VanGuard Pre-column in order to prolong the lifetime of your analytical ACQUITY Column. The lab equipment works in conjunction with your column and connects directly to the inlet of the ACQUITY UPLC Column, which results in minimized extra column volumes and reduces leaking by eliminating additional connections. Compared to traditional C18 phases, the C4 ligand used in the ACQUITY UPLC Protein BEH C4 VanGuard Pre-column is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity. Further, the Waters Protein Separation Technology (PrST) columns are QC tested and optimized to separate proteins based on size, hydrophobicity, and isoelectric point, making the analytical column crucial lab equipment that should be protected from harm.
The pre-column works by protecting your column from damaging contaminants and enhances and improves the column’s analytical performance. This is achieved by filtering out any chemical and particulate impurities that may be present in the mobile phase stream.
The ACQUITY UPLC Protein BEH C4 VanGuard Pre-column is one of the first guard column devices that has been specially designed for routine use and can tolerate pressures up to 18,000 psi (1240 bar) in applications run on the ACQUITY UPLC System. The product listed here contains Waters patented BEH Technology C4, 300Å pore, and 1.7µm particles.
To guarantee the quality of the equipment you receive from us, each batch of the ACQUITY UPLC Protein BEH C4 VanGuard Pre-column is QC tested with a peptide map to make certain that you get consistent performance and increased analytical column life. The pre-column is made using the exact packing material as the analytical columns, so they can be used with the ACQUITY UPLC columns for reversed-phase Liquid Chromatography, and deliver the same performance levels.
The ACQUITY UPLC Protein BEH C4 VanGuard Pre-column is available in multiple pore and particle sizes that you can find in our online store and shop for lab equipment that best suits your needs.
You may also want to explore the ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 75 µm X 100 mm, 1/pk for your lab.
What Does The Expression Of A Protein Mean?
The concept of protein expression encompasses the intricate processes involved in the synthesis, modification, and regulation of proteins within living organisms. In the realm of protein research, the term "protein expression" can refer to both the specific protein under investigation and the laboratory methodologies employed for protein production. It encompasses the diverse aspects of protein biosynthesis, post-translational modifications, and regulatory mechanisms that govern protein function and behavior in biological.