ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 1K - 150K, 1/pk

With Waters ACQUITY UPLC Glycan BEH Amide Column, you can achieve remarkable retention of polar analytes with a wide range of polarity (including carbohydrates and sugars), structural moiety, and pka. Because the lab equipment is designed to retain polar analytes and metabolites that are too polar for reversed-phase (RP) chromatography, they can be used with a wide range of mobile phase pH (2-11).

Our ACQUITY UPLC Glycan BEH Amide Columns have a chemically stable, trifunctionally bonded amide phase based on Waters' innovative ethylene bridged hybrid (BEH) particle technology. This gives HILIC (Hydrophilic Interaction Chromatography) separations a whole new level of stability and adaptability. For compounds of polar and ionizable chemicals, the utilization of unbonded BEH particles enables an orthogonal separation mechanism.

The ACQUITY UPLC BEH Glycan Separation Technology Column, commonly known as BEH Glycan, is designed to separate the released glycans of biopharmaceuticals as their 2-aminobenzamide (2-AB) derivatives using ACQUITY UPLC systems with fluorescence detection (FLR). The lab equipment has been developed and proven to achieve efficient, repeatable separations under UPLC HILIC conditions, overcoming a significant weakness of the HILIC stationary phases, chemical stability.

When compared to reversed-phase materials, the Waters ACQUITY UPLC BEH Amide columns provide enhanced mass spectrometry response, direct compatibility with sample preparation eluates (PPT, LLE, and SPE), as well as orthogonal selectivity.

The ACQUITY UPLC Glycan BEH Amide Columns are ideally suited for carbohydrate analysis and offer numerous advantages. The 1.7 m particle size allows for high-speed, high-resolution analysis in complicated matrices while maintaining or enhancing chromatographic resolution.

The lab equipment increases quantitation accuracy because, unlike amine-based columns, they are not vulnerable to Schiff base formation. The use of high pH and high temperature to collapse reducing sugar anomers, minimize analytical durations, and improve MS detection is possible due to their higher pH stability. The use of BEH technology in conjunction with a trifunctionally bound amide phase extends column lifetime and increases assay robustness. You may also want to explore and purchase the GlycoWorks Rapid Deglycosylation Kit - 1 x 24.