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SKU: 186004742 ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 150K, 1/pk
The ACQUITY UPLC BEH Glycan Separation Technology Column, also known as BEH Glycan, is optimized for use with ACQUITY UPLC systems with fluorescence detection (FLR), separating the released glycans of biopharmaceuticals as their 2-aminobenzamide (2-AB) derivatives.
ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk
Specifically developed and extensively QC tested for superior UPLC component resolution in less time for glycan structures, the ACQUITY UPLC Glycan BEH Amide column works with Waters" ACQUITY UPLC system to achieve column chemistry that is suited ideally to help chromatographers get the correct answers more quickly. Waters" Glycan separation technology is optimized for use with ACQUITY UPLC systems with fluorescence detection, offering improved component resolution in a shorter time than other HPLC-based methods.
Waters" glycan analysis UPLC columns are based on the patented second-generation BEH hybrid particles. With this proprietary bonding process, Waters has developed a robust, amide based, ligand chemistry to be used in the demanding application of glycan separation. Extensive quality control testing is performed against relevant, labeled glycan standards in order to ensure consistent column to column performance and batch to batch. This state of the art bonding procedure for short amine-containing liquid is combined with the particle structure and bonding chemistry that remains stable at low pH and elevated temperature to offer consistent glycan separations.
By combining the ACQUITY UPLC system with BEH technology with this technology for glycan separation, analysis time and cost per sample can be reduced while resolution is improved. By using several complementary analytical techniques, scientists are able to better characterize, identify, and quantitate oligosaccharides isolated from glycoproteins. HLIC is a well recognized, reliable technique that can be effectively used to separate fluorescently-labeled glycan. In combination with Waters" ACQUITY UPLC system, the ACQUITY UPLC Glycan BEH Amide Column gives improved component resolution compared to more conventional LC techniques, offering more certain identification and reliable quantitation.
Each batch of BEH Glycan column material undergoes rigorous testing to ensure consistent and reliable performance. All columns include a Certificate of Analysis which reports physical, chemical, and chromatographic tests obtained from the actual separation of a qualified 1-AB Labeled Human IgG standard.
FAQs About ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 150K, 1/pk
1. What types of glycans can be analyzed with the ACQUITY UPLC Glycan BEH Amide Column?
The ACQUITY UPLC Glycan BEH Amide Column is specifically designed for the analysis of released glycans, making it ideal for profiling N-linked and O-linked glycans. It supports the separation of glycans with molecular weights ranging from 1,000 to 150,000 Daltons, offering high resolution for both small and complex glycan structures.
This column is particularly effective in separating glycan isomers and identifying heterogeneity in glycosylation patterns, making it a critical tool for biopharmaceutical applications, such as glycoprotein characterization and quality control of therapeutic proteins.
2. Is this column compatible with fluorescence or mass spectrometry (MS) detection?
Yes, the ACQUITY UPLC Glycan BEH Amide Column is fully compatible with both fluorescence and mass spectrometry (MS) detection methods. Its design ensures excellent chromatographic performance while maintaining compatibility with commonly used glycan labeling techniques, such as 2-AB and 2-AA labeling for fluorescence detection. The column's high resolution and ability to separate isomers also enhance MS data quality by providing clean baselines and distinct peaks, facilitating accurate identification and quantification of glycans in complex mixtures.
3. How does this column perform under high-temperature conditions?
The ACQUITY UPLC Glycan BEH Amide Column is engineered to perform reliably under elevated temperature conditions, typically up to 80°C. Higher temperatures can improve the separation of complex glycan mixtures by reducing viscosity and enhancing analyte diffusion, leading to sharper peaks and faster run times.
The column's BEH particle technology ensures stability and longevity even under these demanding conditions, making it suitable for workflows that require precise glycan analysis at elevated temperatures. Proper temperature control can further optimize separations and maintain column performance over time.
What are glycans?
Glycan is another term for a polysaccharide, or a compound consisting of a large number of monosaccharides like glycosidically. The term glycan can also be used to refer to the carbohydrate portion of a glycoconjugate.
Glycosylation is one of the most common forms of post-translational modification of human proteins. Glycosylated proteins make up 50-70% of human proteins, while about 30% of the cell surface is made up of glycolipids and glycoproteins.
Glycans, therefore, play critical roles in many physiological and pathological reactions, including immunity, blood clotting, cell development, and death.
What is UPLC?
Ultra Performance Liquid Chromatography (UPLC) represents a groundbreaking advancement in separation science, surpassing the capabilities of High Performance Liquid Chromatography (HPLC). UPLC Columns are meticulously filled with minute particles [1.7 – 1.8 µm], empowering your laboratory to reach unparalleled levels of chromatographic performance. By embracing UPLC Technology, your lab can elevate resolution, sensitivity, and speed to the utmost level, without any compromise on quality or efficiency.
