Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 186004801 ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1/pk
ACQUITY BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. Based on BEH technology the ACQUITY Amide columns offer a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 12 to enable the separation of polar analytes that span a wide range of polarity, structural moiety and pKa.
ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1/pk
Achieve exceptional retention of polar analytes spanning a wide range in polarity (including carbohydrates and sugars), structural moiety and pka with Waters ACQUITY UPLC BEH Amide columns. The columns facilitate the use of a wide range of mobile phase pH (2-11) as they are designed to retain polar analytes and metabolites that are too polar to retain reversed-phase (RP) chromatography.
Enjoy a chemically stable, trifunctionally-bonded amide phase based on Waters novel ethylene bridged hybrid (BEH) particle technology in our ACQUITY UPLC BEH Amide columns. This enables a new dimension in stability and versatility for HILIC (Hydrophilic Interaction Chromatography) separations. The use of unbonded BEH particles also provides orthogonal separation mode for mixtures of polar and ionizable compounds.
Designed to overcome a major weakness of the HILIC stationary phases, chemical stability, the columns are optimized and tested to produce efficient, reproducible separations under UPLC HILIC conditions.
The Waters ACQUITY UPLC BEH Amide columns are excellent lab equipment as they also provide increased mass spectrometry response, direct compatibility with sample preparation eluates (PPT, LLE, and SPE) as well as orthogonal selectivity compared to reversed-phase materials.
The ACQUITY Amide columns are exceptional for the analysis of carbohydrates providing many benefits. The 1.7 µm particle size enables high speed and high-resolution analysis in complex matrices while improving or maintaining chromatographic resolution. The columns improve quantitation accuracy as they are not susceptible to Schiff base formation, unlike the amine-based columns. Their increased pH stability enables the use of high pH and high temperature to collapse reducing sugar anomers, shorten analysis times and improve MS detection. The combination of BEH technology with a trifunctionally-bonded amide phase provides exceptional column lifetime and improves assay robustness.
For periods longer than four days at room temperature, store reversed-phase columns in 100% acetonitrile. For elevated temperature applications, store immediately after use in 100% acetonitrile for the best column lifetime. Do not store columns in buffered eluents. If the mobile phase contained a buffer salt, flush reversed-phase ACQUITY BEH columns with 10-column volumes of HPLC grade water and replace with 100% acetonitrile for storage. Failure to perform this intermediate step could result in precipitation of the buffer salt in the column when 100% acetonitrile is introduced. Run a gradient to 100% ACN in order to flush all aqueous solvent from BEH Amide columns prior to storage in 100% ACN. Completely seal columns to avoid evaporation and drying out of the bed.
