ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk

Get exceptional retention of polar analytes spanning a wide range of polarity, structural moiety, and pKa with the ACQUITY UPLC BEH Amide Column. The columns make this possible by using Waters innovative stationary phases for Hydrophilic Interaction Chromatography (HILIC) that overcome the challenge of retaining and separating extremely polar compounds. The lab equipment is based on ethylene bridged hybrid (BEH) particle technology and leverages a chemically stable, trifunctionally-bonded amide phase that gives additional stability and versatility for HILIC separations.

Compared to reversed-phase materials, the ACQUITY UPLC BEH Amide Columns also offer orthogonal selectivity and direct compatibility with sample preparation eluates (PPT, LLE and SPE). You also benefit from increased mass spectrometry response in addition to the enhanced retention of polar compounds.

The ACQUITY UPLC BEH Amide Columns are outstanding for the analysis of carbohydrates. Their 1.7 µm particle size ensures that high-speed and high-resolution analysis of carbohydrates is achieved in complex sample matrices while improving or maintaining chromatographic resolution, making them ideal lab equipment for the analysis of one of the most abundant and diverse classes of compounds.

Utilize high pH and high temperatures to collapse reducing sugar anomers with the ACQUITY UPLC BEH Amide Columns to shorten analysis time and improve MS detection.

Protect your analytical column with the ACQUITY UPLC BEH Amide VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk. Perfectly compatible with the ACQUITY UPLC BEH Amide Column listed here, the pre-column is lab equipment designed to enhance the lifetime of your column by removing all chemical and particulate contaminants and ensuring you get exceptional results every time. Also browse through our other portfolio items, including other ACQUITY UPLC BEH Amide Columns, and shop for lab equipment as well.

FAQs About ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk

1. What types of compounds are best separated on this column besides polar analytes?
While the ACQUITY UPLC BEH Amide Column is renowned for its ability to separate polar analytes, it is also effective for other compound types. Hydrophilic compounds with strong hydrogen bonding capabilities can be separated efficiently due to the column"s unique selectivity. Additionally, its amide-based stationary phase is particularly useful for resolving isomeric compounds that are challenging to separate on other columns. While not its primary purpose, the column can also handle some basic compounds when used with the appropriate mobile phase additives.

2. Is this column suitable for analyzing peptides or proteins?
The ACQUITY UPLC BEH Amide Column is generally not recommended for peptides or proteins due to the challenges these compounds present. Peptides and proteins often have high molecular weights, which can lead to strong interactions with the stationary phase, causing irreversible adsorption and poor recovery. Furthermore, the mobile phase conditions typically used with this column may result in protein denaturation or degradation.
For peptides and proteins, specialized reversed-phase columns like the ACQUITY UPLC BEH C18 or columns specifically designed for proteins are better suited. These alternatives provide optimal separation and recovery for biomolecules, ensuring reliable and reproducible results.

3. What are the signs of column degradation, and how can I prevent them?
Signs of column degradation include increased backpressure, which may indicate particle bed compaction or contaminant buildup. A loss of resolution, where peaks broaden and separation efficiency decreases, is another key indicator. Changes in retention times and poor peak shapes, such as tailing or fronting, may signal degradation or contamination.
To prevent these issues, store the column upright, avoid extreme temperatures, and never let it dry out. Regularly flush the column with appropriate solvents to remove contaminants. Using a guard column can protect the analytical column by trapping impurities, and filtering the mobile phase ensures particulates don"t damage the stationary phase.