ACQUITY UPLC BEH Amide 1.7µm 1.0x50mm
When compared to reversed-phase materials, the ACQUITY UPLC BEH Amide Column provides better mass spectrometry response, direct compatibility with sample preparation elutes (PPT, LLE, and SPE), and orthogonal selectivity.
The chemically stable, tri-functionally connected amide phase based on Waters' revolutionary ethylene bridged hybrid (BEH) particle technology is available with the ACQUITY UPLC BEH Amide Column. This offers a new degree of stability and versatility to HILIC (Hydrophilic Interaction Chromatography) separations. Unbonded BEH particles in laboratory equipment can enable orthogonal separation of polar and ionizable chemical mixtures. With a novel design that helps to overcome one of the HILIC stationary phases' key weaknesses, chemical stability, the column has been designed and tested to perform efficient, reproducible separations under UPLC HILIC conditions.
Use the ACQUITY UPLC BEH Amide Column to perform superior carbohydrate analysis. The 1.7 µm particle size of the column enables high-speed and high-resolution analysis in complex matrices while boosting or maintaining chromatographic resolution. It also improves quantitation accuracy since it is not susceptible to Schiff base formation, unlike amine-based columns. The increased pH stability of the ACQUITY UPLC BEH Amide Column enables the use of high pH and high temperature to collapse, reducing sugar anomers, shortening analysis times, and improving MS detection.
Add the ACQUITY UPLC BEH Amide Column to your lab equipment collection to get exceptional retention of polar analytes across a wide range of polarity (including carbohydrates and sugars), structural moiety, and PKA. Because it was designed to retain polar analytes and metabolites that are too polar for reversed-phase (RP) chromatography, the column can be utilized with a wide range of mobile phase pH. (2-11). The column's lifetime and assay robustness are enhanced by the combination of BEH technology and a tri-functionally linked amide phase.
Visit our website to shop for lab equipment and contact us with any questions you may have. You may also be interested in ACQUITY UPLC BEH Amide VanGuard Pre-column; Amide columns, such as ACQUITY BEH, hold very polar molecules such as carbohydrates and sugars. The ACQUITY Amide columns, which are based on BEH technology, have a chemically stable, tri-functionally linked amide phase that is stable from pH 2 to 12, allowing them to separate polar analytes with a wide range of polarity, structural moiety, and pKa.
