Chemistry |
Amide |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
11 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Endfitting Type |
Waters |
Pore Size |
130 Å |
Format |
Column |
Surface Area |
185 |
System |
HPLC |
Particle Technology |
BEH |
USP Classification |
L68 |
Inner Diameter |
2.1 mm |
Length |
100 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge BEH Amide Column, 130Å, 3.5 µm, 2.1 mm X 100 mm, 1/pk
Opt for the Waters XBridge BEH Amide column to retain extremely polar compounds, including carbohydrates and sugars. The use of the XBridge Amide analytical column provides you with a chemically stable, trifunctionally bonded amide phase that is stable across a wide pH range, making it possible for you to conduct the separation of polar analytes across a variety of features.
As the XBridge BEH Amide Column is based on Waters’ novel ethylene bridged hybrid (BEH) particle, it makes use of a chemically stable, trifunctionally bonded amide phase to offer unprecedented stability and versatility in HILIC separations lab equipment. Waters has developed this ability as an innovative approach to overcoming the challenge of retaining and separating extremely polar compounds in HILIC stationary phases. The XBridge BEH Amide Column has the same selectivity as ACQUITY UPLC BEH Amide columns. This enables scientists to easily transfer separations seamlessly between HPLC and UPLC technology platforms.
The XBridge BEH Amide Column is easily able to support the use of a wide range of mobile phase pH, from 2 to 11, as it has been designed specifically to retain polar analytes and metabolites that are too polar to retain using reversed-phase chromatography. This results in the exceptional retention of polar analytes spanning a wide range in polarity, structural moiety, and pKa.
The XBridge BEH Amide Column listed here is particularly suited for the analysis of carbohydrates with the 3.5 µm particle size and 130Å pore size. This enables high resolution and high-speed analysis in complex sample matrices while maintaining or improving the chromatographic resolution.
Refer to our product catalog or online store to shop for lab equipment that is authentic and available at the best available rates in the market.
Waters recommends that you also invest in the XBridge BEH Amide VanGuard Cartridge, 130Å, 3.5 µm, 2.1 mm X 5 mm, 3/pk, so that your analytical column can last a long time and continue to deliver at its peak performance throughout its life.
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