Chemistry |
Cation-Exchange |
Separation Mode |
Ion-Exchange |
Particle Substrate |
Polymethacrylate |
pH Range Min |
2 pH |
pH Range Max |
11 pH |
Particle Shape |
Spherical |
Particle Size |
7 µm |
Endfitting Type |
Waters |
Format |
Column |
System |
HPLC |
Inner Diameter |
4.6 mm |
Length |
100 mm |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
Protein-Pak |
Product Type |
Columns |
Units per Package |
1 pk |
Protein-Pak Hi Res SP, 7 µm, 4.6 mm x100 mm, 1/pk
To assist in the characterization of recombinant proteins, monoclonal antibodies, and other biological compounds, Waters has developed a series of columns named the Protein-Pak Hi Res Ion-Exchange (IEX) columns. The lab equipment features particles that have non-porous, high compound binding capacity and yield an outstanding resolution of charged species in less time compared to the use of many traditional porous IEX offerings. In addition to this, to ensure consistent batch-to-batch performance quality, control testing with defined protein standards is conducted, with the results held to stringent levels.
Opt for the use of the Protein-Pak Hi Res SP Column in order to characterize recombinant proteins and monoclonal antibodies that are present in many of the biopharmaceutical therapeutics as well as other biological compounds. The high molecular weight limitations that are encountered with the traditional porous IEX particles are overcome with the non-porous, high-ligand density particles of the Protein-Pak Hi Res SP Column. The Protein-Pak Hi Res SP uses a sulfopropyl ligand.
The Protein-Pak Hi Res SP Column makes it possible for you to get high sample loading capacities and component resolution while minimizing column fouling. This is because the column makes use of a multi-layered network of ion-exchange particles on non-porous particles, which assists in the effective diffusion and binding of charged proteins or other biomolecules onto the particles.
The Protein-Pak Hi Res SP Column provides you with stability in high salt concentrations and standard pressures via the use of polymer-based particles, as well as the ability to perform analysis over a wide pH range.
To ensure that you receive only the top-quality equipment as promised by Waters, we conduct extensive testing on our lab equipment, guaranteeing reliable and consistent results. The Protein-Pak Hi Res Q Column is manufactured at our cGMP, ISO9001, and ISO3485 certified facilities under the strictest conditions, where each step is carefully monitored and controlled, so you have only the highest quality equipment for use.
You may also want to explore and invest in the IEX Anion Exchange Standard, which is a mix that contains 3 proteins, enabling you to benchmark the column when using anion exchange. We recommend that this standard be used on a regular basis for benchmarking column and system performance, similar to the QCRM product line, to ensure that the lab equipment continues to deliver optimal performance.
What Is The pH Range For The Protein-Pak Hi Res SP?
The Protein-Pak Hi Res SP listed here can operate well in the pH limit of 2-11.