Protein-Pak Hi Res Q Column.

SKU: 186004931
Protein-Pak Hi Res Q, 5 µm, 4.6 mm x 100 mm, 1/pk

Protein-Pak Hi Res Q | 186004931


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Product Description

Protein-Pak Hi Res Ion-Exchange (IEX) columns were developed to assist in the characterization of recombinant proteins, monoclonal antibodies, and other biological compounds. The non-porous, high compound binding capacity of these particles yields outstanding resolution of charged species in less time compared to use of many traditional porous IEX offerings. In addition, quality control testing with defined protein standards helps ensure consistent batch-to-batch performance.

Specifications

  • Chemistry

    Anion-Exchange

  • Separation Mode

    Ion-Exchange

  • Particle Substrate

    Polymethacrylate

  • pH Range Min

    2 pH

  • pH Range Max

    11 pH

  • Particle Shape

    Spherical

  • Particle Size

    5 µm

  • Endfitting Type

    Waters

  • QC Tested

    Protein

  • Format

    Column

  • System

    HPLC

  • Inner Diameter

    4.6 mm

  • Length

    100 mm

  • UNSPSC

    41115709

  • Application

    Protein, Gene Therapeutics

  • Brand

    Protein-Pak

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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Protein-Pak Hi Res Q, 5 µm, 4.6 mm x 100 mm, 1/pk

Use the Protein-Pak Hi Res Q Column to characterize recombinant proteins and monoclonal antibodies found in many of the biopharmaceutical therapeutics as well as other biological compounds. The high molecular weight limitations that are presented with the traditional porous, IEX particles are overcome with the non-porous, high-ligand density particles of the Protein-Pak Hi Res Q Column.

You can get high sample loading capacities and component resolution while minimizing column fouling using the Protein-Pak Hi Res Q Column. This is because the column makes use of a multi-layered network of ion-exchange particles on non-porous particles. This assists in the effective diffusion and binding of charged proteins or other biomolecules onto the particles.

Perform analysis over a stable, wide pH range (3-10). The Protein-Pak Hi Res Q Column offers stability in high salt concentrations and standard pressures via the use of polymer-based particles.

In addition to this, extensive quality control testing with defined protein standards is conducted to help ensure consistent batch-to-batch performance, guaranteeing reliable and consistent results. The Protein-Pak Hi Res Q Column is manufactured at our cGMP, ISO9001, and ISO3485 certified facilities under the strictest conditions, where each step is carefully monitored and controlled so you have only the highest quality equipment for use.

Find all the relevant products that work in conjunction with the Protein-Pak Hi Res Q Column, as well as other variants of this listing. You will find ample literature that helps understand the technology used in our products and manufacturing on our website. The website also enables you to shop for lab equipment directly from us.

You may also be interested in Reversed-Phase QC Reference Material, which is a significantly complex reference material mixture containing compounds that are challenging to separate. This mix includes a void marker, neutral, acidic, and basic compounds including uracil, butylparaben, naphthalene, propranolol, dipropyl phthalate, acenaphthene, and amitriptyline at pH 7.

FAQs About Protein-Pak Hi Res Q, 5 µm, 4.6 mm x 100 mm, 1/pk

1.What are the recommended applications for the Protein-Pak Hi Res Q column?
The Protein-Pak Hi Res Q Column is typically used in the analysis and purification of proteins, peptides, nucleic acids, and other charged biomolecules. Its applications include protein purification, biopharmaceutical analysis, and research in proteomics and genomics. The column is suitable for both analytical and preparative scale separations.

2.What is the packing material for this column?
The packing material in the Protein-Pak Hi Res Q Column consists of quaternary ammonium (Q) functional groups bonded to a robust matrix. This anion-exchange material provides strong interaction with negatively charged analytes, facilitating effective separation based on charge differences.

3.What is the recommended temperature range for this column?
Waters recommends using the Protein-Pak Hi Res Q Column at temperatures between 4 °C and 30 °C. Operating within this temperature range ensures stable performance and prolongs the column's lifespan.

4.Is the Protein-Pak Hi Res Q Column suitable for both analytical and preparative applications?
Yes, the Protein-Pak Hi Res Q Column is suitable for both analytical and preparative applications. Its design and particle size allow for high-resolution separations in analytical scale experiments, while its construction and performance characteristics make it capable of handling larger sample loads in preparative scale purifications.

How Is The Protein-Pak Hi Res Q Column Equilibrated?

The Protein-Pak Hi Res Q Column is shipped in 20 mM sodium sulfate with 0.05% sodium azide. It is important to ensure mobile-phase compatibility before changing to a different mobile-phase system. To equilibrate the Protein-Pak Hi Res Q Column, use a minimum of 10 column volumes of the buffer to be used.