ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 4.6 mm X 300 mm, 1/pk

The ACQUITY UPLC Protein BEH SEC Column is a high-performance size-exclusion chromatography (SEC) column designed for the precise separation of proteins based on size. Engineered for use with Waters' ACQUITY Ultra Performance Liquid Chromatography (UPLC) systems, this column delivers fast analysis times, exceptional resolution, and reliable performance for a wide range of protein analysis applications. It is particularly useful for protein characterization, aggregation studies, and purity assessments in biopharmaceutical research and quality control settings.

This column features a 1.7 µm particle size, which enables high-resolution separations and sharp peak definition. The smaller particle size allows for improved efficiency, making it possible to analyze complex protein samples with greater accuracy. The 200 Å pore size is ideal for separating proteins within the molecular weight range of 10,000 to 500,000 Daltons, making it well-suited for studying monoclonal antibodies, enzymes, and other biomolecules commonly encountered in biological and pharmaceutical research.

The column's packing material is based on Waters' bridged ethyl hybrid (BEH) particle technology, which provides outstanding chemical and mechanical stability. This advanced particle composition enhances the durability of the column, ensuring reliable performance over multiple runs while maintaining high sensitivity and reproducibility. The BEH technology also allows for operation over a broad pH range, making it compatible with various mobile phases and experimental conditions. This versatility is crucial for laboratories working with diverse protein formulations.

Designed specifically for UPLC systems, the ACQUITY UPLC Protein BEH SEC Column leverages the high-pressure capabilities of these instruments to enable faster separations without compromising resolution. Compared to traditional HPLC SEC columns, this column significantly reduces analysis time while maintaining the integrity of protein separations. The ability to perform rapid, high-quality separations improves laboratory efficiency, making it an ideal choice for research facilities that require both speed and accuracy in their workflows.

The column dimensions of 4.6 mm x 300 mm offer a standard format for analytical SEC separations, providing a balance between resolution and sample capacity. The internal diameter of 4.6 mm ensures compatibility with common flow rates used in UPLC applications, while the 300 mm length enhances separation performance by allowing for improved resolution of protein species. This column is sold as a single unit, making it easy to integrate into existing laboratory setups.

With its high efficiency, rapid analysis capabilities, and robust performance, the ACQUITY UPLC Protein BEH SEC Column is an essential tool for researchers and analysts conducting protein separations. Designed for seamless integration with Waters' ACQUITY UPLC systems, it offers the reliability and precision needed for demanding protein research, biopharmaceutical development, and quality control applications.

FAQs about ACQUITY UPLC Protein BEH SEC Column, 200Å

What applications is the ACQUITY UPLC Protein BEH SEC Column, 200Å specifically designed for?
The ACQUITY UPLC Protein BEH SEC Column, 200Å is engineered for the analysis of proteins and their aggregates, providing high-resolution size exclusion chromatography and being particularly suited for monoclonal antibody and protein biotherapeutic analysis.

How does the pore size of the ACQUITY UPLC Protein BEH SEC Column, 200Å affect its separation capabilities?
The 200Å pore size of this column is optimized for the separation of small to medium-sized proteins, enabling it to effectively resolve proteins based on their hydrodynamic volume, which is crucial for accurate molecular sizing.

Can the ACQUITY UPLC Protein BEH SEC Column, 200Å be utilized for both qualitative and quantitative analysis?
Yes, the ACQUITY UPLC Protein BEH SEC Column, 200Å is suitable for both qualitative and quantitative analysis, providing reproducible retention times and peak areas essential for detailed characterization and precise quantification of proteins.

What type of sample preparation is required before using the ACQUITY UPLC Protein BEH SEC Column, 200Å?
Sample preparation for the ACQUITY UPLC Protein BEH SEC Column, 200Å typically involves ensuring the sample is in an appropriate buffer and is filtered or centrifuged to remove particulates that could clog the column.

Is the ACQUITY UPLC Protein BEH SEC Column, 200Å compatible with various detection techniques?
Yes, the ACQUITY UPLC Protein BEH SEC Column, 200Å is compatible with a range of detection techniques including UV, fluorescence, and mass spectrometry, making it a versatile tool for comprehensive protein analysis.