SKU: 186005226
ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 4.6 mm X 300 mm, 1/pk

Acquity UPLC Protein BEH SEC Column | 186005226

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Product Description

Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 4.6 mm X 300 mm represents the first commer­cially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 10000 to 450,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods.   Note:  The same column is also available with the BEH200 SEC Protein Standard at no additional cost. (PN: 176003905)   Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Specifications

  • Chemistry

    SEC

  • Separation Mode

    SEC

  • Particle Substrate

    Hybrid

  • pH Range Min

    2.5 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    60 C

  • Maximum Pressure

    10000 psi (690 Bar)

  • Endcapped

    No

  • Bonding Technology

    SEC

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    10000

  • Molecular Weight Range Max

    450000

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    200 Å

  • QC Tested

    Protein

  • Format

    Column

  • Packing Solvent

    20% methanol in water

  • Surface Area

    220

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L33

  • Inner Diameter

    4.6 mm

  • Length

    300 mm

  • Carbon Load

    12 %

  • eCord

    Yes

  • UNSPSC

    41115709

  • Application

    Protein

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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FAQs about ACQUITY UPLC Protein BEH SEC Column, 200Å

What applications is the ACQUITY UPLC Protein BEH SEC Column, 200Å specifically designed for?
The ACQUITY UPLC Protein BEH SEC Column, 200Å is engineered for the analysis of proteins and their aggregates, providing high-resolution size exclusion chromatography and being particularly suited for monoclonal antibody and protein biotherapeutic analysis.

How does the pore size of the ACQUITY UPLC Protein BEH SEC Column, 200Å affect its separation capabilities?
The 200Å pore size of this column is optimized for the separation of small to medium-sized proteins, enabling it to effectively resolve proteins based on their hydrodynamic volume, which is crucial for accurate molecular sizing.

Can the ACQUITY UPLC Protein BEH SEC Column, 200Å be utilized for both qualitative and quantitative analysis?
Yes, the ACQUITY UPLC Protein BEH SEC Column, 200Å is suitable for both qualitative and quantitative analysis, providing reproducible retention times and peak areas essential for detailed characterization and precise quantification of proteins.

What type of sample preparation is required before using the ACQUITY UPLC Protein BEH SEC Column, 200Å?
Sample preparation for the ACQUITY UPLC Protein BEH SEC Column, 200Å typically involves ensuring the sample is in an appropriate buffer and is filtered or centrifuged to remove particulates that could clog the column.

Is the ACQUITY UPLC Protein BEH SEC Column, 200Å compatible with various detection techniques?
Yes, the ACQUITY UPLC Protein BEH SEC Column, 200Å is compatible with a range of detection techniques including UV, fluorescence, and mass spectrometry, making it a versatile tool for comprehensive protein analysis.

What is BEH Technology?

BEH Technology, a pivotal component of UPLC advancements, is characterized by the utilization of 1.7 µm BEH (Ethylene Bridged Hybrid) particles. These particles come in various pore sizes and bonded phases, enabling the application of both reversed-phase and hydrophilic interaction chromatography. This technology finds relevance in the analysis of small molecules as well as large molecule biopharmaceuticals. Notably, the hybrid particle technology possesses inherent chemical stability, thereby offering a wider pH range (pH 1-12). This remarkable feature renders the technology versatile and robust for method development, ensuring reliable separations. The BEH particle technology is available in multiple HPLC particle sizes (2.5, 3.5, 5, and 10 µm), facilitating a seamless transition between HPLC and UPLC technology platforms. This flexibility allows laboratories to adapt their chromatographic approaches while capitalizing on the benefits offered by BEH particles.

What is UPLC?

UPLC, which stands for Ultra Performance Liquid Chromatography, epitomizes the cutting-edge evolution in separation science, surpassing the traditional boundaries set by HPLC (High Performance Liquid Chromatography). Distinctive UPLC columns, meticulously filled with smaller particles ranging from 1.7 to 1.8 µm, unlock an extraordinary realm of chromatographic performance for your laboratory. With UPLC Technology at your disposal, expect nothing less than remarkable enhancements in resolution, sensitivity, and speed, all achieved without any compromises. Prepare to embark on a transformative journey that redefines the limits of liquid chromatography.