ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1/pk

When you are searching for lab equipment that provides excellent peak shape, high efficiency, and loading capacity for basic compounds while using acidic, low ionic strength mobile phases, the ACQUITY UPLC CSH C18 columns are designed for your needs. Offering alternate selectivity compared to other reversed-phase UPLC columns, these are ultra-efficient and overcome peak shape asymmetry and poor loading capacity for basic compounds, column bleed, and rapid mobile phase re-equilibration. Methods development scientists in all applications can benefit from the widest range of selectivities available in any modern LC columns.

These columns feature Waters" new and innovative Charged Surface Hybrid (CSH) technology, enabling increased loading capacity and rapid column re-equilibration after changing mobile phase pH. CSH Technology begins with the ethylene bridged hybrid (BEH) particle, which is rugged and efficient, and then applies controlled surface charge to add reproducible low-level charge to the particle surface. This is then functionalized with the appropriate bonded phase chemistry, including bonding and end-capping. This transformative technology combination offers superior peak shape for base compounds, improved batch to batch reproducibility, and excellent stability at low pH.

CSH Technology provides TFA peak shape performance without the use of TFA and its associated disadvantages. Instead, improved peak shape performance is achieved using an MS-friendly mobile phase additive such as formic acid, which does not exhibit the lingering chromatographic effects of an ion-pairing agent. This facilitates straightforward and faster method development.

All ACQUITY CSH packing materials are created specifically for use with the ACQUITY UPLC System and all manufacturing is done in a cGMP, ISO 9001 certified manufacturing facility, using ultra-pure reagents. Each batch is tested chromatographically with acidic, basic, and neutral analytes, with results held to narrow specification ranges that ensure excellent, reproducible performance. Optimize confidence in your results every time by also utilizing the Neutrals QC Reference Material in a wide variety of separation methods.

ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1/pk FAQs

How does Charged Surface Hybrid (CSH) technology improve performance?
Charged Surface Hybrid (CSH) technology enhances chromatographic performance by incorporating a low-level positive surface charge onto Waters' Hybrid Particle Technology. This modification significantly improves peak shape, particularly for basic compounds, which often exhibit tailing on traditional C18 columns. By reducing secondary interactions between the stationary phase and ionizable analytes, CSH technology results in sharper peaks and improved reproducibility.

Another advantage is enhanced retention control, allowing for better separation of closely eluting compounds. The increased interaction between analytes and the stationary phase improves resolution, even under high-throughput UPLC conditions. Additionally, CSH particles offer excellent mechanical and chemical stability, making them ideal for longer column lifetimes and robust performance across a wide range of mobile phase conditions.

What is the optimal pH range for this column?
The ACQUITY UPLC CSH C18 Column is designed to operate within a pH range of 1 to 11, making it highly versatile for a wide variety of analytical applications. This broad range allows for the use of both acidic and basic mobile phases, making it possible to fine-tune separations based on the chemical properties of the analytes.

For acidic conditions (pH 1-4), formic acid, trifluoroacetic acid (TFA), or acetic acid are commonly used to improve peak shape and enhance ionization efficiency for LC-MS applications. In higher pH conditions (pH 7-11), buffers like ammonium bicarbonate or ammonium acetate help maintain the stability of basic compounds while ensuring high-resolution separations.

The column's Charged Surface Hybrid (CSH) particle technology enhances stability at higher pH levels, unlike traditional silica-based C18 columns, which degrade in alkaline conditions. This durability allows for greater method flexibility, making the column an excellent choice for laboratories handling a wide range of sample types.