Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Endcapped |
Yes |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
30000 |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
300 Å |
QC Tested |
Peptide |
Format |
Column |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
1 mm |
Length |
100 mm |
Carbon Load |
18 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC Peptide BEH C18 Column, 300Å, 1.7 µm, 1 mm X 100 mm, 1K - 30K, 1/pk
Utilize the ACQUITY UPLC Peptide BEH C18 Column to improve peptide chromatography. It will help you achieve compact, symmetrical peaks for the best resolution and successfully separate a variety of peptides, including hydrophobic and hydrophilic, big and small, acidic and basic. Formic acid and trifluoroacetic acid (TFA) provide good peak shapes and retention for effective chromatography and detection.
The Waters Peptide Separation Technology (PST), which uses BEH Technology particles, is the foundation for the ACQUITY UPLC Peptide columns, which are specially QC validated with a peptide map and meet the stringent requirements for the BEH Technology particles. The durability of well-developed peptide separations and predictable behavior with a variety of samples utilized in proteomics, protein characterization, and peptide synthesis is ensured by doing this. Sharp, symmetrical peaks are produced over a broad range of peptide characteristics and application requirements thanks to the surface chemistry.
The large pore (300 ) and tri-functional bonding of the Waters ACQUITY UPLC Peptide BEH C18 Columns make them special. These columns provide remarkable low pH stability and a broad, usable pH range (1–12). They provide extremely low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis, making them essential pieces of lab equipment for any scientist.
The ACQUITY UPLC Peptide BEH C18 Columns are developed in a cGMP, ISO 9002 accredited plant using ultra-pure reagents and are specifically designed to work with ACQUITY UPLC and ACQUITY PREMIER systems. Each batch is qualified for peptide mapping after undergoing chromatographic testing with neutral, acidic, and basic analytes. The results are held to limited specification ranges to ensure reproducibility and consistency, batch over batch and column over column, so that you receive superb lab equipment that offers reliable analysis and reproducible performance.
If you are interested in reviewing additional product offerings from Waters, please review our catalog or check out our website, from where you can directly shop for lab equipment. You should also review the ACQUITY UPLC Peptide BEH C18 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm, 1K - 30K, 3/pk; VanGuard pre-columns are the first guard column devices designed for routine use at pressures up to 15000 psi (1030 bar) in applications run on the ACQUITY UPLC System. This specific offering is specifically QC tested with a peptide map to help ensure consistent performance and increased analytical column life.
Should I Employ An Auto-Sampler?
Although autosamplers and robotic workstations enable automated chromatographic separation and offer reproducible injections, it is important to keep in mind that despite their benefits, they will also significantly increase the cost of your system.