Chemistry |
PFP/Fluoro-Phenyl |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
No |
Bonding Technology |
PFP |
Silanol Activity |
High |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
100 Å |
Format |
Column |
Surface Area |
230 |
System |
UHPLC, UPLC |
Particle Technology |
HSS |
USP Classification |
L43 |
Inner Diameter |
3 mm |
Length |
100 mm |
Carbon Load |
7 % |
eCord |
Yes |
UNSPSC |
41115709 |
Brand |
XSelect |
Product Type |
Columns |
Units per Package |
1 pk |
XSelect HSS PFP Column, 100Å, 2.5 µm, 3 mm X 100 mm, 1/pk
Due to a complex interplay of retention processes, the XSelect HSS PFP column, a low coverage, non-end-capped pentafluorophenyl phase, offers superior separation of chemically similar compounds as well as alternate selectivity to conventional alkyl chain columns. A seamless transition path to UPLC adoption is made possible by the inclusion of 2.5 m eXtended Performance [XP] columns in ultra-low dispersion hardware, which provides superior separation performance, resilience, and throughput for HPLC tests.
One of the most reliable, repeatable, and scalable silica-based PFP LC columns on the market is the XSelect HSS PFP Column. Without compromising peak shape or retention time variation, the distinctive HSS substrate and PFP ligand combination offer real PFP performance. High Strength Silica (HSS) technology is used in the XSelect HSS PFP Column. With advantages like enhanced polar molecule retention and various selectivity, this technology was developed with chromatography in mind. In addition to its analytical capabilities, the HSS particle has the mechanical strength required to sustain UPLC pressures.
The XSelect HSS PFP Column provides a special phase for selectivity for bases and planar aromatic compounds. When you require higher compound retention, especially for basic polar analytes, whose retention is challenging with C18 stationary phases, rely on the XSelect HSS PFP Column. PFP phases are electron acceptors; hence these ligands will interact pi-pi with Lewis base-containing molecules. Based on analyte form, dipole moment, and hydrogen bonding interactions, the stiff aromatic ring offers enhanced selectivity.
The XSelect HSS PFP Column is available in different particle sizes and column formats to help you efficiently develop methods. You can browse through our website to shop for lab equipment and review the full list of Waters' product catalog.
You might also be interested in XSelect HSS PFP XP VanGuard Cartridge, 100 Å, 2.5 µm, 2.1 mm X 5 mm, 3/pk. XSelect HSS PFP VanGuard Cartridges are used to extend analytical column lifetime and performance by removing particulate contamination from the mobile phase stream. This cartridge is optimized to protect all 2.1 mm and 3.0 mm I.D. XSelect HSS PFP analytical columns containing 2.5 µm sorbent particles.
How Is Enrichment In Sample Components Present In Low Concentration Achieved?
To enrich sample components that are present in low concentrations, several steps can be taken. To selectively elute analytes, you can modify the gradient steps. Utilizing "huge" sample quantities in an adsorption-promoting solvent is another efficient technique. Use a "small" collecting volume in a solvent that encourages desorption. Utilize sorbent chemistry specific to the analyte, rather than the chemistry found in the analytical column. It is important to carefully select the chemistry of the solid-phase extraction column to eliminate the need for additional sample preparation.