SKU: 186006505
ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm X 150 mm, 1/pk

ACQUITY UPLC Protein BEH SEC Columns | 186006505

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Product Description

Based upon the highly successful BEH particle technology, the  ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm X 150 mm represents the first commer­cially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 1000 to 80,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods.   Note:  The same column is also available with the BEH125 SEC Protein Standard at no additional cost. (PN: 176003906)  Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Specifications

  • Chemistry

    SEC

  • Separation Mode

    SEC

  • Particle Substrate

    Hybrid

  • pH Range Min

    2.5 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    60 C

  • Maximum Pressure

    10000 psi (690 Bar)

  • Endcapped

    No

  • Bonding Technology

    SEC

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    1000

  • Molecular Weight Range Max

    80000

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    125 Å

  • QC Tested

    Protein

  • Format

    Column

  • Packing Solvent

    20% methanol in water

  • Surface Area

    220

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L33

  • Inner Diameter

    4.6 mm

  • Length

    150 mm

  • Carbon Load

    12 %

  • UNSPSC

    41115709

  • Application

    Protein

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm X 150 mm, 1K - 80K, 1/pk

Calculate the aggregate determinations for molecular weights between 1000- 80,000 Daltons faster than traditional SEC assays with the ACQUITY UPLC Protein BEH SEC Column. The analytical column has been optimized for the analysis of proteins and their aggregates to offer robust, fast, and accurate determinations.

Get exceptional improvements in resolution, throughput, and sensitivity for assays with the ACQUITY UPLC Protein BEH SEC Column. The column enables you to monitor the presence of protein aggregates through size-exclusion chromatography (SEC), thus increasing the efficacy since the presence of these proteins can compromise the safety and efficacy of biologic-based therapeutics.

The packing materials used in the ACQUITY UPLC Protein BEH SEC Column were designed specifically for use with the ACQUITY UPLC System. The ACQUITY UPLC Protein BEH SEC Columns are manufactured in a cGMP, ISO 9001 certified plant using ultra-pure reagents. In addition to this, each batch of the packing material is tested chromatographically with acidic, basic, and neutral analytes. The results from these tests are held to narrow specification ranges to assure excellent, reproducible performance. Every ACQUITY UPLC Protein BEH SEC Column is individually tested and a Performance Chromatogram and Certificate of Batch Analysis are provided on the eCord Intelligent Chip so you can rely on the quality of your lab equipment and can be certain that you will get consistent results.

Enhance the life of your analytical column by using the ACQUITY UPLC Protein BEH SEC Guard Column, 125Å, 1.7 µm, 4.6 mm X 30 mm, 1K - 80K, 1/pk. The Guard column has been designed to work specifically with the ACQUITY UPLC Protein BEH SEC Column and extends the lifetime of the column by removing any non-desired sample matrix or particulates onto the column without degrading the separation. It can be easily replaced to restore separation performance and extend the analytical column’s lifetime.

Check our catalog to find all related products and other variants of the same listing as well as our complete range of products, and also to shop for lab equipment.

What Is The pH Range For ACQUITY UPLC Protein BEH SEC Column?

It is recommended that the ACQUITY UPLC Protein BEH SEC Column be operated in the pH range of 1–12. In addition to this, the column’s lifetime will vary depending upon the operating temperature, the type, and concentration of buffer used.