SKU: 186006506
ACQUITY UPLC Protein BEH SEC Column, 125 Å, 1.7 µm, 4.6 mm X 300 mm, 1/pkg

ACQUITY UPLC Protein BEH SEC Columns | 186006506

Streamline Purchasing with Customized Quotes

Streamline Purchasing with Customized Quotes

Shop on Waters.com and add items to your cart

Navigate to the Shopping Cart

Select Request a Quote to get your quote instantly

Learn More

Product Description

Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm X 300 mm represents the first commer­cially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 1000 to 80,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods.   Note:  The same column is also available with the BEH125 SEC Protein Standard  at no additional cost. (PN: 176003907)  Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Specifications

  • Chemistry

    SEC

  • Separation Mode

    SEC

  • Particle Substrate

    Hybrid

  • pH Range Min

    2.5 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    60 C

  • Maximum Pressure

    7000 psi (483 Bar)

  • Endcapped

    No

  • Bonding Technology

    SEC

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    1000

  • Molecular Weight Range Max

    80000

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    125 Å

  • QC Tested

    Protein

  • Format

    Column

  • Packing Solvent

    20% methanol in water

  • Surface Area

    220

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L33

  • Inner Diameter

    4.6 mm

  • Length

    300 mm

  • Carbon Load

    12 %

  • UNSPSC

    41115709

  • Application

    Protein

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

What do you want to do?

What do you want to do?
Need Help?

Related Products

Back To Top Back To Top

ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm X 300 mm, 1K - 80K, 1/pk

With the ability to rapidly separate and accurately quantitate protein or peptide monomers from their respective aggregates, ACQUITY UPLC Protein BEH SEC Columns are chosen by biopharmaceutical chromatographers across the globe. These columns are enabled by Waters’ unique Ethylene-Bridged Hybrid (BEH) Diol coated particle technology, allowing accurate measurements of aggregation species up to 10 times faster and with less eluent than traditional SEC methods that use soft gels or >5µm rigid particles. This allows a significant reduction in the requirement for high salt concentration mobile phases to decrease non-desired, ionic secondary interactions between protein/peptide and SEC particle surface.

As a well-established and USP/EP approved method for the analysis of undesired protein or peptide aggregates from active monomeric forms, SEC separations have traditionally used HPLC technology. By performing separations using Waters UPLC-based lab equipment, chromatographers can achieve comparatively improved component resolution while also reducing both analysis time and mobile phase consumption. This allows products to be moved to market faster while still offering the consistency required by drug regulatory bodies. The ACQUITY UPLC Protein BEH SEC Column and ACQUITY UPLC Protein BEH SEC Guard Column, 125Å, 1.7 µm, 4.6 mm X 30 mm, 1K - 80K, 1/pk have unique surface chemistry particles that significantly reduce secondary interactions common in SEC, leading to less need for aggressive mobile-phase salt concentrations.

All ACQUITY UPLC SEC columns are quality control tested using relevant proteins and peptides to ensure superior consistency across batches and giving you increased confidence in proven and validated methods. All products are synthesized using high-quality raw materials and manufactured in ISO-certified manufacturing facilities.

What Is The Difference Between A Peptide And A Protein?

Both proteins and peptides are fundamental components of cells that carry out critical biological functions. Proteins give shape to cells and respond to signals transmitted from the surrounding environment, while peptides can play a key role in regulating other molecules’ activity. The two are very similar, both made up of chains of amino acids and held together by peptide bonds. The main distinction is that peptides are much smaller than proteins, typically containing 2-50 amino acids, while proteins are made up of over 50 amino acids. Peptides also tend to have a less defined structure than proteins, which can adopt complex formations called secondary, tertiary, and quaternary structures.