Chemistry |
Amide |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
11 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
130 Å |
Format |
Column |
Surface Area |
185 |
System |
HPLC |
Particle Technology |
BEH |
USP Classification |
L68 |
Inner Diameter |
2.1 mm |
Length |
100 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge BEH Amide Column, 130Å, 5 µm, 2.1 mm X 100 mm, 1/pk
Add the Waters XBridge BEH Amide Column to your lab in order to be able to maintain very polar molecules, including carbohydrates and sugars. Easily conduct the separation of polar analytes across a number of properties like polarity, structural moiety, and pKa by utilizing the XBridge Amide analytical column, which provides you with a chemically stable, tri-functionally linked amide phase that is stable throughout a wide pH range of 2-11.
The XBridge BEH Amide Column is based on Waters' innovative ethylene bridged hybrid (BEH) particle and uses a chemically stable, tri-functionally bound amide phase to provide exceptional stability and versatility in HILIC separations lab equipment. This capability was created by Waters as a novel solution to the problem of maintaining and separating very polar chemicals in HILIC stationary phases. The XBridge BEH Amide Column has the same selectivity as ACQUITY UPLC BEH Amide columns, which makes it possible for scientists to easily transfer separations seamlessly between HPLC and UPLC technology platforms.
The lab equipment can easily handle the usage of a wide range of mobile phase pH since it was built expressly to retain polar analytes and metabolites that are too polar to retain using reversed-phase chromatography. With a particle size of 3.5 m and a pore size of 130, this listing is perfect for the analysis of carbohydrates. You may achieve high resolution and high-speed analysis in complicated sample matrices using the XBridge BEH Amide Column while preserving or improving chromatographic resolution.
If you would like to see more lab equipment from Waters, you can look through our catalog or visit our website, where you can shop for lab equipment to meet all of your laboratory needs. You may want to review the XBridge BEH Amide VanGuard Cartridge, 130Å, 5 µm, 2.1 mm X 5 mm, 3/pk; XBridge BEH Amide VanGuard Cartridges are used to extend analytical column lifetime and performance by removing particulate contamination from the mobile phase stream. This cartridge is optimized to protect all 2.1 mm and 3.0 mm I.D. XBridge BEH Amide analytical columns containing 5 µm sorbent particles.
What Is The Significance Of BEH Within Chromatography?
One of the primary enablers underlying UPLC technology is the BEH (Ethylene Bridged Hybrid) particle, which comes in a variety of pore sizes and bonded phases, allowing for both reversed-phase and hydrophilic contact chromatography. The approach may be used to evaluate a variety of biopharmaceutical compounds, including tiny and big molecules.