Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 186006725 XBridge BEH Amide Column, 130Å, 2.5 µm, 3 mm X 150 mm, 1/pk
XBridge BEH Amide XP Column | 130Å | 2.5 µm | 3 mm X 150 mm | 1/pk | 186006725
XBridge BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. XBridge Amide columns offer you a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 11 to enable the separation of polar analytes spanning a wide range of polarity, structural moiety and pKa. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.
XBridge BEH Amide XP Column, 130Å, 2.5 µm, 3 mm X 150 mm, 1/pk
Based on Waters’ novel ethylene bridged hybrid (BEH) particle, XBridge BEH Amide XP Columns utilize a chemically stable, trifunctionally bonded amide phase that has enabled a new dimension of stability and versatility in HILIC separations. By offering the same selectivity as ACQUITY UPLC BEH Amide columns, scientists are able to use these columns to transfer separations between HPLC and UPLC technology platforms.
These columns have been designed to retain polar analytes and metabolites that are too polar to retain through reversed-phase chromatography, facilitating the use of a wide range of mobile phase (2-11) to enable to exceptional retention of polar analytes spanning a wide range in polarity, structural moiety, and pKa. In addition, XBridge BEH Amide XP Columns provide increased mass spectrometry response, direct compatibility with sample preparation eluates (PPT, LLE, and SPE), along with orthogonal selectivity compared to reversed-phase materials. This allows HILIC to be an attractive alternative separation technique.
As the most abundant and diverse class of compounds analyzed through HILIC, a range of carbohydrates (monosaccharides, polysaccharides, disaccharides, oligosaccharides) are able to be analyzed using XBridge BEH Amide XP Columns. The increased chemical stability allows for the use of high pH and high temperatures to collapse anomers without the loss of reducing sugars. Unlike amine-based lab equipment, these columns are not susceptible to Schiff base formation, improving quantitation accuracy.
What Information Is Included With XBridge BEH Amide XP Columns?
Each column includes a Certificate of Analysis specific to the batch of packing material, including the gel batch number, analysis of unbonded particles, and chromatographic results and conditions. A Performance Test Chromatogram is also included, which is specific to each individual column, including the gel batch number, column serial number, USP plate count, USP tailing factor, retention factor, and chromatographic test conditions.
These data points should be recorded and stored for future reference, or they can be accessed via the ACQUITY UPLC console.
