The iKey Separation device is one of the enabling technologies behind the ionKey/MS system. The innovative iKey eliminates the need for traditional fittings and greatly simplifies the microflow-LC user experience. Available with an internal diameter of 150 µm and in 50 mm and 100 mm lengths, the iKey brings microflow LC into the mainstream of chromatographic laboratories.
Chemistry |
C4 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
10 pH |
Maximum Pressure |
10000 psi (690 Bar) |
Endcapped |
No |
Silanol Activity |
Medium |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Pore Size |
300 Å |
QC Tested |
Protein |
Format |
Separation Device |
Surface Area |
90 |
System |
Nano/Micro |
Particle Technology |
BEH |
USP Classification |
L26 |
Inner Diameter |
150 µm |
Length |
50 mm |
Carbon Load |
8 % |
UNSPSC |
41115854 |
Application |
Protein |
Brand |
iKey |
Product Type |
Columns |
Units per Package |
1 pk |
iKey Protein BEH C4 Separation Device, 300Å, 1.7 µm, 150 µm X 50 mm, 1/pk
The iKey Separation Device comes with the ionKey/MS System and includes all of the necessary fluid connections, electronics, ESI interface, column heater, eCord, and chemistry to perform UPLC separations. The iKey Separation device replaces traditional fittings and columns with a single unit, simplifying the user experience. These devices are designed to be a "plug and play" solution, eliminating the user-dependent fluctuation in findings that is common in traditional microflow LC-MS examinations.
The ionKey/MS Systems were created with the goal of integrating UPLC separation into the mass spectrometer's source and providing remarkable simplicity of use and performance. Users using ionKey/MS benefit from a 40x increase in sensitivity, a simplified user experience, the capacity to do repeated studies with tiny sample quantities, the ability to examine smaller sizes, and reduced solvent consumption during analyses. When compared to analytical scale LC-MS analysis, the iKey Peptide BEH C18 Separation Device provides good peak shape, boosts peak height, and enhances S:N.
Browse through our website to find other products and collections of Waters products, and shop for lab equipment as per your laboratory requirements. Our website also allows you to contact a member of our global support team. Our customer service professionals can help you with any questions or problems you may have, as well as assisting you in selecting the finest solutions for your laboratory and chromatographic needs.
We also recommend checking out the Reversed-Phase QC Reference Material; the Reversed-Phase QC Reference Material contains seven compounds, including uracil, butylparaben, naphthalene, propranolol, dipropyl-phthalate, acenaphthene, and amitriptyline at pH 7. This standard mix should be used to confirm the benchmark performance of your analytical HPLC or UPLC system. This particular QC Reference Material is a specially formulated mix that includes a void marker, neutral, acidic, and basic compounds.
What Are The Limitations Of The iKey Peptide BEH C18 Separation Device In Terms Of Pressure And pH?
This iKey Peptide BEH C18 Separation Device can resist pressures up to 10,000 psi (or 690 bar) and a pH range of 1 to 10, but we recommend that it be used within certain parameters. When lab equipment is exposed to severe pressures, pH, and/or temperatures, its lifetime and effectiveness are reduced.
What Does It Mean To Have A High Resolution In Chromatography?
Peak width and retention time, both inherent features of the chromatographic process, are used to calculate chromatography resolution. Resolution measurements can be used to improve separations, and they are routinely done so. The higher the resolution, the better the results can be examined and the more accurate they can be.