SKU: 186006852
ACQUITY UPLC Protein BEH SEC Column, 450 Å, 2.5 µm, 4.6 mm x 300 mm, 1/pk

ACQUITY UPLC Protein BEH SEC Column | 186006852

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Product Description

Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 450 Å, 2.5 µm, 4.6 mm x 300 mm is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods.

Note: This column is also shipped with the BEH450 SEC Protein Standardat no additional cost at no additional cost (PN: 176002997) . Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Specifications

  • Chemistry

    SEC

  • Separation Mode

    SEC/GPC

  • Particle Substrate

    Hybrid

  • pH Range Min

    2.5 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    60 C

  • Maximum Pressure

    5000 psi (345 Bar)

  • Endcapped

    No

  • Bonding Technology

    SEC

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    100000

  • Molecular Weight Range Max

    1500000

  • Particle Shape

    Spherical

  • Particle Size

    2.5 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    450 Å

  • QC Tested

    Protein

  • Format

    Column

  • Packing Solvent

    20% methanol in water

  • Surface Area

    80

  • System

    UPLC

  • Particle Technology

    BEH

  • Technique

    LC

  • USP Classification

    L33

  • Inner Diameter

    4.6 mm

  • Length

    300 mm

  • Carbon Load

    9 %

  • eCord

    Yes

  • UNSPSC

    41115709

  • Application

    Protein

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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ACQUITY UPLC Protein BEH SEC Column, 450Å, 2.5 µm, 4.6 mm X 300 mm, 100K - 1M, 1/pk

The ACQUITY UPLC Protein BEH SEC Column uses the highly successful BEH particle technology and is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons. Including the ACQUITY UPLC Protein BEH SEC Column, the total system solution will routinely deliver fast, accurate aggregate determinations of proteins up to 10 times quicker than traditional HPLC-based SEC methods. The patent-pending ACQUITY UPLC Protein BEH SEC Column set a new industry standard for protein analysis by being the first commercially available in the sub 2µm SEC chemistry.

Using the ACQUITY UPLC Protein BEH SEC Column, you can deliver exceptional improvements in resolution, throughput, and sensitivity for assays. The lab equipment makes it possible for you to easily monitor the presence of protein aggregates through size-exclusion chromatography (SEC). As the presence of these proteins can compromise the safety and efficacy of biologic-based therapeutics, the ability to monitor the protein aggregates increases the efficacy of analysis.

The ACQUITY UPLC Protein BEH SEC Column is ideally suited to work in conjunction with the ACQUITY UPLC System as its packing materials were designed specifically for this purpose. Additionally, the column is manufactured to the highest standards in a cGMP, ISO 9001 certified plant using ultra-pure reagents. To ensure that it continues to deliver outstanding performance, each batch of the packing material is tested chromatographically with acidic, basic, and neutral analytes. The results from these tests are held to narrow specification ranges to assure excellent, reproducible performance. Further, every ACQUITY UPLC Protein BEH SEC Column is individually tested, and a Performance Chromatogram and Certificate of Batch Analysis are provided on the eCord Intelligent Chip so you can rely on the quality of your lab equipment and can be certain that you will get consistent results.

Shop for lab equipment and check all compatible products and other variants of the product listed here as well as our complete range of lab equipment from our website or catalog.

You may also want to explore the BEH450 SEC Protein Standard Mix and other related products.

How Is A Specific Protein Separated?

In order to separate the protein from the cells where it is present, it is necessary to isolate the cells by centrifugation. In particular, centrifugation using media with different densities may be useful to isolate proteins expressed in specific cells.