Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
11 pH |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
15000 |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Direct-Connect |
Pore Size |
130 Å |
QC Tested |
Peptide |
Format |
VanGuard Pre-column |
Surface Area |
185 |
System |
UHPLC, UPLC |
Particle Technology |
CSH |
USP Classification |
L1 |
Inner Diameter |
2.1 mm |
Length |
5 mm |
Carbon Load |
15 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
ACQUITY UPLC |
Product Type |
Guards |
Units per Package |
3 pk |
What Are The Best Techniques For Isolating And Cleaning Analytes?
Two primary solid-phase extraction (SPE) methods are commonly employed to separate and purify target components in samples. The first method, known as retention-cleanup-elution, is utilized when the concentrations of the target chemicals are too low for reliable quantification. This approach involves retaining the analytes, cleaning up the sample matrix, and subsequently eluting the concentrated analytes for further analysis. It allows for the concentration of dilute samples and enrichment of trace chemicals. On the other hand, when the target component is present in high concentrations, a pass-through cleanup method can be chosen. In this approach, the sample is passed through the SPE sorbent to remove interfering substances, but no enrichment of the target component occurs.