SKU: 186006939
ACQUITY UPLC Peptide CSH C18 VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 1K - 15K, 3/pk


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Product Description

Waters ACQUITY UPLC CSH130 C18 1.7 µm particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. The fact that the performance of a CSH130 C18 column exhibits little dependence on strong ion pairing agents makes it ideal for LC or LC-MS applications. The VanGuard pre-column connects directly to the inlet of an eXtended Performance column which minimizes extra columns volumes and reduces leaking by eliminating additional connections.

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    11 pH

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    1000

  • Molecular Weight Range Max

    15000

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Direct-Connect

  • Pore Size

    130 Å

  • QC Tested

    Peptide

  • Format

    VanGuard Pre-column

  • Surface Area

    185

  • System

    UHPLC, UPLC

  • Particle Technology

    CSH

  • USP Classification

    L1

  • Inner Diameter

    2.1 mm

  • Length

    5 mm

  • Carbon Load

    15 %

  • UNSPSC

    41115709

  • Application

    Peptide

  • Brand

    ACQUITY UPLC

  • Product Type

    Guards

  • Units per Package

    3 pk

Product Support

Documents

Documents



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Two primary solid-phase extraction (SPE) methods are commonly employed to separate and purify target components in samples. The first method, known as retention-cleanup-elution, is utilized when the concentrations of the target chemicals are too low for reliable quantification. This approach involves retaining the analytes, cleaning up the sample matrix, and subsequently eluting the concentrated analytes for further analysis. It allows for the concentration of dilute samples and enrichment of trace chemicals. On the other hand, when the target component is present in high concentrations, a pass-through cleanup method can be chosen. In this approach, the sample is passed through the SPE sorbent to remove interfering substances, but no enrichment of the target component occurs.