Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
11 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
15000 |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Endfitting Type |
Waters |
Pore Size |
130 Å |
QC Tested |
Peptide |
Format |
Column |
Surface Area |
185 |
System |
HPLC |
Particle Technology |
CSH |
USP Classification |
L1 |
Inner Diameter |
4.6 mm |
Length |
150 mm |
Carbon Load |
15 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
XSelect |
Product Type |
Columns |
Units per Package |
1 pk |
XSelect Peptide CSH C18 Column, 130Å, 3.5 µm, 4.6 mm X 150 mm, 1K - 15K, 1/pk
Waters XSelect CSH130 C18 3.5 µm particles have a low and precisely defined positive charge concentration, resulting in a fairly good peak shape for peptides utilizing either FA or TFA-containing mobile phases. The low reliance on strong ion pairing agents in the performance of a CSH130 C18 column makes it appropriate for LC or LC-MS applications.
The Waters Charged Surface Hybrid (CSH) technology enables the column to produce superior peak form and enhanced basic compound loading capacity. The CSH130 C18 chemistry's ability to absorb higher peptide mass loading than many other columns will improve the ability to detect potentially essential low-level constituents along with the main components of interest. Using either 0.1 percent FA or 0.1 percent TFA containing eluents, this column delivers remarkable peak capabilities in HPLC, UPLC, or LC-MS separations of peptides and small proteins up to 10,000 Daltons.
You may achieve scalable chemistries with XSelect Peptide CSH C18 Column, from nanoflow analysis to lab-scale purification, as well as excellent mass loading of complicated peptide samples for peptide mapping, proteomics applications, or analysis and lab-scale purification of synthetic peptides. This particular analytical column is operational in the pH range of 1 to 11, up to a maximum pressure of 6000 psi (or 415 Bar), and has a Molecular Weight Range between 1,000 to 15,000.
If you would like to review additional equipment offered by Waters, please review our brochure or visit our website to shop for lab equipment to meet the needs of your laboratory. You may also be interested in checking out LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk; All Waters LCMS Certified vials are produced following tightly controlled manufacturing processes and handling procedures. They are tested with mass spectrometers and come packaged with a certificate of analysis showing the reference and vial scan for the manufacturing lot.
What Are The Best Recommended Techniques For Isolating And Cleaning Analytes?
For separating and cleaning up sample components of interest, two general SPE methods are used. When the concentrations of the chemicals of interest are too low for accurate and precise quantification, a retention-cleanup-elution approach is typically used. This strategy allows for the concentration of dilute samples as well as trace enrichment of chemicals. When the desired sample component is present at a high concentration, a pass-through cleanup approach may be selected. When a pass-through cleanup approach is applied, however, no sample enrichment occurs.