SKU: 186006957
XSelect Peptide CSH C18 Column, 130Å, 3.5 µm, 4.6 mm X 150 mm, 1K - 15K, 1/pk

XSelect Peptide CSH C18 Column | 186006957


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Product Description

Waters XSelect CSH130 C18 3.5 µm particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. The fact that the performance of a CSH130 C18 column exhibits little dependence on strong ion pairing agents makes it ideal for LC or LC-MS applications.

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    11 pH

  • Maximum Pressure

    6000 psi (415 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    1000

  • Molecular Weight Range Max

    15000

  • Particle Shape

    Spherical

  • Particle Size

    3.5 µm

  • Endfitting Type

    Waters

  • Pore Size

    130 Å

  • QC Tested

    Peptide

  • Format

    Column

  • Surface Area

    185

  • System

    HPLC

  • Particle Technology

    CSH

  • USP Classification

    L1

  • Inner Diameter

    4.6 mm

  • Length

    150 mm

  • Carbon Load

    15 %

  • UNSPSC

    41115709

  • Application

    Peptide

  • Brand

    XSelect

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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XSelect Peptide CSH C18 Column, 130Å, 3.5 µm, 4.6 mm X 150 mm, 1K - 15K, 1/pk

Waters XSelect CSH130 C18 3.5 µm particles have a low and precisely defined positive charge concentration, resulting in a fairly good peak shape for peptides utilizing either FA or TFA-containing mobile phases. The low reliance on strong ion pairing agents in the performance of a CSH130 C18 column makes it appropriate for LC or LC-MS applications.

The Waters Charged Surface Hybrid (CSH) technology enables the column to produce superior peak form and enhanced basic compound loading capacity. The CSH130 C18 chemistry's ability to absorb higher peptide mass loading than many other columns will improve the ability to detect potentially essential low-level constituents along with the main components of interest. Using either 0.1 percent FA or 0.1 percent TFA containing eluents, this column delivers remarkable peak capabilities in HPLC, UPLC, or LC-MS separations of peptides and small proteins up to 10,000 Daltons.

You may achieve scalable chemistries with XSelect Peptide CSH C18 Column, from nanoflow analysis to lab-scale purification, as well as excellent mass loading of complicated peptide samples for peptide mapping, proteomics applications, or analysis and lab-scale purification of synthetic peptides. This particular analytical column is operational in the pH range of 1 to 11, up to a maximum pressure of 6000 psi (or 415 Bar), and has a Molecular Weight Range between 1,000 to 15,000.

If you would like to review additional equipment offered by Waters, please review our brochure or visit our website to shop for lab equipment to meet the needs of your laboratory. You may also be interested in checking out LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk; All Waters LCMS Certified vials are produced following tightly controlled manufacturing processes and handling procedures. They are tested with mass spectrometers and come packaged with a certificate of analysis showing the reference and vial scan for the manufacturing lot.

What Are The Best Recommended Techniques For Isolating And Cleaning Analytes?

For separating and cleaning up sample components of interest, two general SPE methods are used. When the concentrations of the chemicals of interest are too low for accurate and precise quantification, a retention-cleanup-elution approach is typically used. This strategy allows for the concentration of dilute samples as well as trace enrichment of chemicals. When the desired sample component is present at a high concentration, a pass-through cleanup approach may be selected. When a pass-through cleanup approach is applied, however, no sample enrichment occurs.