Separation Mode |
SEC/GPC |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
8 pH |
Endcapped |
No |
Silanol Activity |
Medium |
Molecular Weight Range Min |
200 |
Molecular Weight Range Max |
5000 |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
45 Å |
Format |
Column |
System |
APC |
Particle Technology |
BEH |
Technique |
SEC |
Inner Diameter |
4.6 mm |
Length |
150 mm |
eCord |
Yes |
UNSPSC |
41115709 |
Brand |
ACQUITY APC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY APC AQ BEH Column, 45Å, 1.7 µm, 4.6 mm X 150 mm, 200 - 5K, 1/pk
ACQUITY® Advanced Polymer Chromatography® (APCTM) Columns are equipped with high-performance chemistries that enable rapid and accurate chromatographic characterization of synthetic polymer and macromolecular species. When compared to existing polymer characterization methods, the unique hybrid-polymer sub-3-µm particle technology outperforms conventional polymeric-based phases in terms of chromatographic efficiency, stability, and method adaptability. ACQUITY APC Columns provide exceptional chromatographic performance for all polymer classes, including aqueous and organic soluble polymers with low molecular weight.
ACQUITY APC AQ columns are developed to enable highly efficient size-based separations for characterization of water-soluble polymers. The sorbent employs patented Ethylene Bridge Hybrid (BEH) technology to create a mechanically robust packed bed that resists shrinking and swelling, allowing for simple mobile phase solvent switching. This column's molecular weight range is estimated to be between 200 and 5,000 g/mole.
ACQUITY APC Columns deliver unrivaled separation speed, resolution, and chromatographic efficiency when compared to conventional polymer-based stationary phases due to improved pore volume and particle size. The laboratory equipment creates a stable bed, which prevents the shrinking and swelling that occurs with polymeric stationary phases when the solvent is switched. The analytical column is produced in the Waters chromatography chemistry manufacturing plant under the most stringent cGMP, ISO 9002 requirements. From raw material characterization through final batch analysis, every step of our column manufacturing process is monitored and closely managed to ensure unrivaled product reproducibility.
Visit our website to shop for lab equipment to learn more about the ACQUITY APC AQ column variants or to find more goods that work with it; you may browse through our website, refer to our catalog, and ensure your lab needs are addressed as needed. You might also be interested in the Polyethylene Glycol Standards Kit, which is a one-of-a-kind collection of ten GPC standards (Gel Permeation Chromatography standards) for determining aqueous molecular weight. Polyethylene Glycol Standards Kit includes ten 1.0g vials with the following molecular weight standards: 100, 200, 400, 600, 1000, 1500, 4300, 7000, 13000, and 22000.
Why Might A Gradient System Be Necessary?
In order to enhance chromatographic separations, gradient systems offer the flexibility to adjust flow rates and introduce solvent/buffer changes. These systems can effectively refine separations and expedite the re-equilibration process of the column. For method development purposes, a four-solvent gradient system comprising methanol, acetonitrile, ammonium acetate buffer, and formic acid solution proves beneficial. On the other hand, quality control laboratories often utilize isocratic systems for swift separations, employing a mobile phase of constant composition that is premixed.