Chemistry |
Amide |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
11 pH |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
150000 |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
130 Å |
QC Tested |
Glycan |
Format |
Column |
Surface Area |
185 |
System |
UHPLC |
Particle Technology |
BEH |
USP Classification |
L68 |
Inner Diameter |
2.1 mm |
Length |
150 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Application |
Glycan |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Glycan BEH Amide XP Column, 130Å, 2.5 µm, 2.1 mm X 150 mm, 1K - 150K, 1/pk
In order to be able to retain extremely polar compounds, including carbohydrates and sugars, depend on the Waters XBridge Glycan BEH Amide XP Column. The lab equipment makes it possible to easily conduct the separation of polar analytes across a variety of features such as polarity, structural moiety, and pKa. The use of the XBridge Glycan BEH Amide XP Column gives you access to a chemically stable, trifunctionally bonded amide phase that is stable across a wide pH range of 2-11.
To help ensure batch-to-batch consistency, as well as highly similar separated glycan profiles, each batch of the BEH Glycan material is specifically quality control tested with the 2-AB labeled Waters Glycan Performance. In addition to this, to make sure that you receive highly similar results, chromatographers can now choose the most appropriate LC-based technology to address their specific released glycan analysis application needs and laboratory instrumentation. The Waters BEH-based Glycan chemistry offerings are available in three highly scalable particle sizes that address UPLC (i.e., 1.7 µm) and HPLC-based (2.5 µm XP and 3.5 µm) application needs. You can learn more about the lab equipment using our online store, which also allows you to purchase them directly from us.
As the XBridge Glycan BEH Amide XP Column is based on Waters’ novel ethylene bridged hybrid (BEH) particle, it makes use of a chemically stable, trifunctionally bonded amide phase and is able to offer unprecedented stability and versatility in HILIC separations lab equipment. This ability was developed by Waters as an innovative approach to overcome the challenge of retaining and separating extremely polar compounds in HILIC stationary phases. In addition to this, the XBridge Glycan BEH Amide XP Column has the same selectivity as ACQUITY UPLC BEH Amide columns, which makes it possible for scientists to easily transfer separations seamlessly between HPLC and UPLC technology platforms.
We recommend that you also explore and purchase the XBridge BEH Amide XP VanGuard Cartridge, 130Å, 2.5 µm, 2.1 mm X 5 mm, 3/pk to protect your column.
What Is The Maximum Pressure That The XBridge Glycan BEH Amide XP Column Can Sustain?
The XBridge Glycan BEH Amide XP Column, mentioned in the provided information, exhibits exceptional durability and can withstand high pressures up to a maximum of 18,000 psi (1240 Bar). This robustness ensures reliable performance and allows for precise separations in demanding liquid chromatography applications. By withstanding such high pressures, the column maintains its integrity and provides accurate and reproducible results for glycan analysis.