ACQUITY UPLC M-Class Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation for biomarker discovery and protein and peptide identification and characterization. All M-Class columns are designed for 15 kpsi (1,000 bar) operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity.
This specific offering contains Waters patented HSS Technology 100 Å pore, T3, 1.8um particles. The mechanically tolerant High Strength Silica [HSS] particle technology was developed to compliment Waters Hybrid Particle Technology [BEH and CSH]. HSS allows for silica based LC separations under UPLC conditions. The particle technology provides the highest retentivity for polar compounds and metabolites while keeping in the retention between polar and hydrophobic analytes balanced.
The higher retention may result in enhanced sensitivity due to improved desolvation as polar analytes elute in a higher percentage organic mobile phase.
The T3 bonding utilizes a trifunctional C18 Alkyl phase bonded at a ligand density that promotes polar compound retention and aqueous mobile phase compatibility.
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Temperature Limits |
45 C |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
Yes |
Bonding Technology |
T3 |
Silanol Activity |
Medium |
Particle Shape |
Spherical |
Particle Size |
1.8 µm |
Endfitting Type |
Valco(male)|360um Open |
Pore Size |
100 Å |
Format |
Column |
Surface Area |
230 |
System |
Nano/Micro |
Particle Technology |
HSS |
USP Classification |
L1 |
Inner Diameter |
75 µm |
Length |
150 mm |
Carbon Load |
11 % |
UNSPSC |
41115709 |
Brand |
ACQUITY UPLC M-Class |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC M-Class HSS T3 Column, 100Å, 1.8 µm, 75 µm X 150 mm, 1/pk
For biomarker discovery and protein identification and characterization, the ACQUITY UPLC M-Class Columns are developed for nano- to microscale liquid chromatographic separation with the best sensitivity, resolution, and reproducibility. Because the lab equipment is so good at controlling pressure changes during injection, it lasts a long time. In order to achieve the best performance, it is necessary to build appropriate connections to avoid peak tailing and poor efficiency.
Using a single chromatographic method, investigate a number of different and complicated formulation compositions using the ACQUITY UPLC M-Class HSS T3 Column. For decades, Waters has been at the forefront of cutting-edge research, bringing cutting-edge solutions to market and meeting all of your analytical needs. You can develop a complete lab equipment portfolio by browsing our website and becoming familiar with our whole product line.
In reversed-phase liquid chromatography, the ACQUITY UPLC M-Class HSS T3 Column enhances the retention of polar compounds and metabolites. Due to low c18 lab equipment, analytes can more easily reach the material's pore structure. This results in balanced retention of polar and hydrophobic molecules without the usage of ion-pair reagents.
The ACQUITY UPLC M-Class HSS T3 Column uses the Waters HSS (High Strength Silica) particle technology, which was engineered to withstand UPLC pressures using a mechanically tolerant, silica-based material. The Waters HSS (High Strength Silica) particle technology uses a revolutionary synthetic approach that improves silica's mechanical stability while maintaining pore volumes that are comparable to those seen in HPLC silica-based materials. This results in higher retentivity when compared to hybrid particle technology. Furthermore, the HSS is an ideal substrate for creating varied selectivity stationary phases.
The HSS particle substrate is trifunctionally attached to the ACQUITY UPLC M-Class HSS T3 Column, allowing for good peak shapes and pH stability. This is provided in addition to better retention when compared to hybrid-based C18 columns. With the exceptional characteristics of the ACQUITY UPLC M-Class HSS T3 Column, you can not only meet the challenges of the laboratory, but you can also be confident that your lab will be more productive and successful in accomplishing your goals.
You may also want to explore the MassPREP Enolase Digestion Standard.
What Is The Carbon Load Of The ACQUITY UPLC M-Class HSS T3 Column?
The ACQUITY UPLC M-Class HSS T3 Column listed here has a carbon load of 11 %.