SKU: 186007474
ACQUITY UPLC M-Class HSS T3 Column, 100Å, 1.8 µm, 75 µm X 250 mm, 1/pk

ACQUITY UPLC M-Class HSS T3 Column | 100Å | 1.8 µm | 75 µm X 250 mm | 1/pk | 186007474

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Product Description

ACQUITY UPLC M-Class Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation for biomarker discovery and protein and peptide identification and characterization. All M-Class columns are designed for 15 kpsi (1,000 bar) operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity.
This specific offering contains Waters patented HSS Technology 100 Å pore, T3, 1.8um particles. The mechanically tolerant High Strength Silica [HSS] particle technology was developed to compliment Waters Hybrid Particle Technology [BEH and CSH].  HSS allows for silica based LC separations under UPLC conditions. The particle technology provides the highest retentivity for polar compounds and metabolites while keeping in the retention between polar and hydrophobic analytes balanced.
The higher retention may result in enhanced sensitivity due to improved desolvation as polar analytes elute in a higher percentage organic mobile phase.
The T3 bonding utilizes a trifunctional C18 Alkyl phase bonded at a ligand density that promotes polar compound retention and aqueous mobile phase compatibility.

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Silica

  • pH Range Min

    2 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    45 C

  • Maximum Pressure

    15000 psi (1034 Bar)

  • Endcapped

    Yes

  • Bonding Technology

    T3

  • Silanol Activity

    Medium

  • Particle Shape

    Spherical

  • Particle Size

    1.8 µm

  • Endfitting Type

    Valco(male)|360um Open

  • Pore Size

    100 Å

  • Format

    Column

  • Surface Area

    230

  • System

    Nano/Micro

  • Particle Technology

    HSS

  • USP Classification

    L1

  • Inner Diameter

    75 µm

  • Length

    250 mm

  • Carbon Load

    11 %

  • UNSPSC

    41115709

  • Brand

    ACQUITY UPLC M-Class

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

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ACQUITY UPLC M-Class HSS T3 Column, 100Å, 1.8 µm, 75 µm X 250 mm, 1/pk

The ACQUITY UPLC M-Class HSS T3 Columns are top-of-the-line columns designed for optimal performance for nano-to-micro scale liquid chromatographic separation. By using the ACQUITY UPLC M-Class HSS T3 Column, you can get a single chromatographic method to analyze a number of different difficult sample compositions. This low-ligand C18 equipment enables the analytes to readily access the pore structure of the material, leading to a balanced retention of polar and hydrophobic molecules without the need for any ion-pair reagents.

The column enables you to easily transfer methods while improving productivity, while also maintaining or increasing the resolution between the components. The silica-based bonded phase used for the ACQUITY UPLC M-Class HSS T3 Column sorbents is compatible with 100% aqueous mobile phase and should be used as a preferred choice when you are looking to develop separations for polar and non-polar compounds. This equipment exhibits ultra-low MS bleed and is fully scalable and transferable to HPLC separations.

The ACQUITY UPLC M-Class HSS T3 Column uses the Waters HSS (High Strength Silica) technology, which has been formulated to withstand UPLC pressures using a mechanically tolerant, silica-based material. The particle technology behind the Waters HSS is an innovative synthetic process that is able to significantly increase the mechanical stability of silica while maintaining pore volumes similar to that of HPLC silica-based materials. The use of this technology means that you get increased retentivity compared to hybrid particle technology, and the HSS serves as the ideal substrate to create stationary phases that offer alternate selectivity.

The superb qualities of the ACQUITY UPLC M-Class HSS T3 Column will enable you to achieve all desired results from your samples and provide you with results you can rely on and count on.

To learn more about the variation of the same product, such as the one listed here, or to find different products that work with it or even to shop for lab equipment so you can replenish your stock and add to your existing equipment portfolio, you can browse our website and refer to our catalog.

You may also want to look into MassPREP Peptide Mixture, which can be used for the evaluation of HPLC columns and systems dedicated to peptide separations.

What Kind Of Pressure Can The ACQUITY UPLC M-Class HSS T3 Column Sustain?

All M-Class columns are designed for 15000 psi (or 1000 Bar) operating pressure and can handle any pressure fluctuations during injection with ease. These traits enhance the column lifetime and longevity.