ACQUITY UPLC M-Class Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. As the 75µm to 180µm ID columns are fritted with a novel frit technology, they are able to withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters patented BEH Technology 130A pore, C18, 1.7um particles. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c to before being QC accepted as a Peptide separation column.
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
10 pH |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Valco(male)|360um Open |
Pore Size |
130 Å |
QC Tested |
Peptide |
Format |
Column |
Surface Area |
185 |
System |
Nano/Micro |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
75 µm |
Length |
250 mm |
Carbon Load |
18 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
ACQUITY UPLC M-Class |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC M-Class Peptide BEH C18 Column, 130Å, 1.7 µm, 75 µm X 250 mm, 1/pk
The ACQUITY UPLC M-Class Column is manufactured to exacting specifications and fitted with state-of-the-art equipment. Due to the chemistry of the column and these strict specifications, it can withstand high pressures. Additionally, it has a high tolerance for pressure changes during injection, increasing longevity.
Because it is made for nano- to micro-scale liquid chromatographic separation, the ACQUITY UPLC M-Class Column should be added to your lab. The lab equipment offers the best sensitivity, resolution, and repeatability for the discovery of biomarkers and the identification and characterization of proteins. It is possible to use the lab equipment with the nanoACQUITY UPLC® Systems.
To achieve outstanding performance, it is essential to build sufficient connections to prevent peak tailing and low efficiency. This is important because, in contrast to regular flow chromatography, capillary-flow systems sometimes call for more constant attention to detail. This solution offers remarkable performance thanks to the utilization of Water's distinctive 130 pores, C18, 1.7 mm particles. Each batch of the packing material used in the lab equipment's manufacturing is QC tested using a gradient separation of a tryptic digest of cytochrome c before being accepted as a Peptide separation column.
Purchase the lab equipment with the assurance that you will only receive top-quality equipment because every ACQUITY UPLC M-Class Column is carefully examined to ensure that it satisfies stringent quality standards. The column is produced in a thoroughly monitored and controlled Waters facility that has received ISO 9001 certification.
Visit our website to learn more about our extensive product catalog and which items work best with the ACQUITY UPLC M-Class Column. You can buy lab equipment there as well.
You may also be interested in the MassPREP Peptide Mixture. A void volume marker and nine carefully chosen peptides with a wide variety of polarities and isoelectric points are included in the MassPREPTM peptide standard mixture. Under the reversed-phase HPLC conditions frequently used for peptide separations, all peaks are easily resolved. The MassPREPTM Peptide Standard Mixture is excellent for testing HPLC peptide separation systems and columns.
What pH Range Is Offered By The ACQUITY UPLC M-Class Peptide BEH C18 Column?
The ACQUITY UPLC M-Class Peptide BEH C18 Column listed here is able to work well across the 1-10 pH range.
What Is The Maximum Pressure That The ACQUITY UPLC M-Class Peptide BEH C18 Column Can Sustain?
This particular ACQUITY UPLC M-Class Peptide BEH C18 Column is able to tolerate a maximum of 15000 psi (1034 Bar) pressure.