ACQUITY UPLC M-Class Trap Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation for biomarker discovery and protein and peptide identification and characterization. All M-Class columns are designed for 15 kpsi (1,000 bar) operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity.
The 2G Trap Columns are designed to minimize peak broadening while maximizing chromatographic efficiency. They can be used in a variety of 1D (low pH) nano flow applications. For 2D applications where the first dimension utilizes high pH, Waters recommends the use of 2D trap columns. This specific offering contains Waters Symmetry 100 Å pore, C18, 5 µm particles. Symmetry silica particles offer on average the highest retention for peptides and are thus most suitable for trapping applications.
The 2G V/V trap columns connect with two V-detail fittings to the main injection valve. Due to their design, 2G trap columns are suitable for bi-directional flow.
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters(male)/Waters(male) |
Pore Size |
100 Å |
Format |
Trap Column |
Surface Area |
335 |
System |
Nano/Micro |
Particle Technology |
Silica |
USP Classification |
L1 |
Inner Diameter |
180 µm |
Length |
20 mm |
Carbon Load |
18 % |
UNSPSC |
41115709 |
Brand |
ACQUITY UPLC M-Class |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC M-Class Symmetry C18 Trap Column, 100Å, 5 µm, 180 µm x 20 mm, 2G, V/V, 1/pk
The ACQUITY UPLC M-Class Trap Columns are made to perform as well as possible in liquid chromatographic separation on a nano- to microscale for the identification and characterization of proteins and peptides as well as the development of new biomarkers. All M-Class columns are built to withstand working pressures of 15 kpsi (1,000 bar) and handle pressure variations during injection very well, resulting in a greater lifetime.
The 2G Trap Columns are made to maximize chromatographic effectiveness while minimizing peak broadening. Numerous 1D (low pH) nano flow applications can make use of them. Waters advises using 2D trap columns in cases where the first dimension uses a high pH. Particles with a Waters Symmetry 100 pore, C18, 5 m size are present in this particular product. The highest retention for peptides is often provided by symmetry silica particles, which makes them ideal for trapping purposes. Two V-detail fittings link the main injection valve to the 2G V/V trap columns. 2G trap columns are appropriate for bi-directional flow due to their architecture.
All of our lab equipment is manufactured under strict conditions and subjected to stringent quality assurance standards, ensuring that you only receive high-quality products from your lab. Our website has columns, guards, vials, and elution plates to meet all of your analytical needs. We provide a variety of brands and versions that have been specifically designed to meet the needs of every type of chromatographic analysis.
The best lab equipment will help you work more productively. Check out our shop for lab equipment; you can browse through our website, refer to our catalog, and make sure your lab needs are met as needed. You can learn more about the different variations of the same product, such as the Waters End Connector Kit, or find additional products that work with it there.
You may also be interested in MassPREP E. coli Digest Standard: The Waters Protein Expression System's MassPREP E. coli digestion standard (MPDS E. coli) is designed to offer a complex biological matrix for creating samples used to train new users. MPDS E. coli was created by using sequencing-grade trypsin to digest a pure E. coli cytosolic protein fraction. The MPDS mixture is purified and free of trypsin, undigested protein, salts, and other substances with high hydrophilicity.
From The Perspective Of Liquid Chromatography, What Does The Term HPLC Stand For?
High-performance liquid chromatography, or HPLC, is an analytical method for separating, identifying, and quantifying mixture components. This test is employed for a number of applications and is the single most often utilized procedure in laboratories worldwide.